Quantitative mass spectrometry evaluation of human retinol binding protein 4 and related variants

Kiernan, Urban A; Phillips, David A; Trenchevska, Olgica; Nedelkov, Dobrin

doi:10.1371/journal.pone.0017282

Quantitative mass spectrometry evaluation of human retinol binding protein 4 and related variants

Journal

PloS one

Date Issued

2011-03-30

Author(s)

Kiernan, Urban A

Phillips, David A

Nedelkov, Dobrin

DOI

10.1371/journal.pone.0017282

Abstract

Retinol Binding Protein 4 (RBP4) is an exciting new biomarker for the determination of insulin resistance and type 2 diabetes. It is known that circulating RBP4 resides in multiple variants which may provide enhanced clinical utility, but conventional immunoassay methods are blind to such differences. A Mass Spectrometric immunoassay (MSIA) technology that can quantitate total RBP4 as well as individual isoforms may provide an enhanced analysis for this biomarker.