Ultrasound influence on coleoptile length at Poaceae seedlings as valuable criteria in prebreeding and breeding processes
Journal
Genetika
Date Issued
2012
Author(s)
Kratovalieva, Suzana
Srbinoska, Marija
Meglic, Vladimir
Andjelkovic, Violeta
DOI
10.2298/gensr1203561k
Abstract
<jats:p>The study includes research on the effect of ultrasound on the ability of
seed germination and coleoptile development of cereal landraces (fam.
Poaceae): oat (Avena sativa L.) brodski, rye (Secale cereale L.) rakotinska,
Triticale svetinikolsko and soft wheat (Triticum aestivum L.) govrlevska. The
experiment with ultrasound was carried out at frequency of 30-40 kHz on the
thermostatic ultrasonic bath. Seeds were sonicated at a constant temperature
(25?C) for 15 min. Ultrasound effect is reflected in the length of the
coleoptyl and mesocotyl, although in a good deal is genetically
predetermined. Coleoptile length was the longest in Triticale (3.3-5.5 cm)
and approximately the same lengths are evident in rye (3.0-6.1 cm).
Concerning the mesocotyl the longest one (5.525 cm ? 0.697) is recorded at
rye, even significantly longer than control of oat and Triticale. Longer
coleoptile of sonicated seeds indicates faster seedling development, good
water supply and rapid development and emergence of first leaves. Correlation
coefficient showed very high (0.821) and high R2 (67,472%) dependence among
variables both, in length of coleoptiles (as dependent variable) and
mesocotyl (as independent) with low standard error (0.225). With the simple
use of ultrasound the germination period could be shorten, water supply more
efficient, the sowing periods will be shorten and good yields even under
conditions of climate change with increased temperatures could be achieved.</jats:p>
seed germination and coleoptile development of cereal landraces (fam.
Poaceae): oat (Avena sativa L.) brodski, rye (Secale cereale L.) rakotinska,
Triticale svetinikolsko and soft wheat (Triticum aestivum L.) govrlevska. The
experiment with ultrasound was carried out at frequency of 30-40 kHz on the
thermostatic ultrasonic bath. Seeds were sonicated at a constant temperature
(25?C) for 15 min. Ultrasound effect is reflected in the length of the
coleoptyl and mesocotyl, although in a good deal is genetically
predetermined. Coleoptile length was the longest in Triticale (3.3-5.5 cm)
and approximately the same lengths are evident in rye (3.0-6.1 cm).
Concerning the mesocotyl the longest one (5.525 cm ? 0.697) is recorded at
rye, even significantly longer than control of oat and Triticale. Longer
coleoptile of sonicated seeds indicates faster seedling development, good
water supply and rapid development and emergence of first leaves. Correlation
coefficient showed very high (0.821) and high R2 (67,472%) dependence among
variables both, in length of coleoptiles (as dependent variable) and
mesocotyl (as independent) with low standard error (0.225). With the simple
use of ultrasound the germination period could be shorten, water supply more
efficient, the sowing periods will be shorten and good yields even under
conditions of climate change with increased temperatures could be achieved.</jats:p>