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http://hdl.handle.net/20.500.12188/11751
Title: | Characterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitro | Authors: | Bartkova, Alexandra Morovic, Martin Strejcek, Frantisek Murin, Matej Benc, Michal Percinic, Florina Popovska Laurincik, Jozef |
Issue Date: | 24-Nov-2020 | Journal: | Animal reproduction | Abstract: | Traditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological assessment of oocyte quality, the assessment based on Lissamine Green B (LB) staining and the analysis of oocytes using a proteomic approach. We evaluated the effectiveness of electrochemical and chemical parthenogenetic activation under our laboratory conditions and evaluated the applicability of Lissamine Green B staining of cumulus-oocyte complexes (COCs) as a non-invasive method for predicting the maturational and developmental competence of porcine oocytes cultured in vitro. We determined that chemical parthenogenetic activation using ionomycin and 6-dimethylaminopurine was slightly more effective than electrochemical activation. After oocyte selection according to LB staining, we found significant differences (P<0.05) between the LB- group and LB+ group and the control group in their maturation, cleavage rate and rate of blastocysts. Proteomic analyses identified a selection of proteins that were differentially expressed in each group of analysed oocytes. Oocytes of the LB- group exhibited an increased variability of proteins involved in transcription regulation, proteosynthesis and the protein folding crucial for oocyte maturation and further embryonic development. These results found a better competence of LB- oocytes in maturation, cleavage and ability to reach the blastocyst stage. | URI: | http://hdl.handle.net/20.500.12188/11751 | DOI: | 10.1590/1984-3143-AR2020-0533 |
Appears in Collections: | Faculty of Veterinary Medicine: Journal Articles |
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