Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/11751
DC FieldValueLanguage
dc.contributor.authorBartkova, Alexandraen_US
dc.contributor.authorMorovic, Martinen_US
dc.contributor.authorStrejcek, Frantiseken_US
dc.contributor.authorMurin, Matejen_US
dc.contributor.authorBenc, Michalen_US
dc.contributor.authorPercinic, Florina Popovskaen_US
dc.contributor.authorLaurincik, Jozefen_US
dc.date.accessioned2021-04-08T11:49:58Z-
dc.date.available2021-04-08T11:49:58Z-
dc.date.issued2020-11-24-
dc.identifier.urihttp://hdl.handle.net/20.500.12188/11751-
dc.description.abstractTraditional methods for the evaluation of oocyte quality are based on morphological classification of the follicle, cumulus-oocyte complex, polar body and meiotic spindle. This study is focused on the differences between the morphological assessment of oocyte quality, the assessment based on Lissamine Green B (LB) staining and the analysis of oocytes using a proteomic approach. We evaluated the effectiveness of electrochemical and chemical parthenogenetic activation under our laboratory conditions and evaluated the applicability of Lissamine Green B staining of cumulus-oocyte complexes (COCs) as a non-invasive method for predicting the maturational and developmental competence of porcine oocytes cultured in vitro. We determined that chemical parthenogenetic activation using ionomycin and 6-dimethylaminopurine was slightly more effective than electrochemical activation. After oocyte selection according to LB staining, we found significant differences (P<0.05) between the LB- group and LB+ group and the control group in their maturation, cleavage rate and rate of blastocysts. Proteomic analyses identified a selection of proteins that were differentially expressed in each group of analysed oocytes. Oocytes of the LB- group exhibited an increased variability of proteins involved in transcription regulation, proteosynthesis and the protein folding crucial for oocyte maturation and further embryonic development. These results found a better competence of LB- oocytes in maturation, cleavage and ability to reach the blastocyst stage.en_US
dc.language.isoenen_US
dc.relation.ispartofAnimal reproductionen_US
dc.titleCharacterization of porcine oocytes stained with Lissamine Green B and their developmental potential in vitroen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.1590/1984-3143-AR2020-0533-
dc.identifier.volume17-
dc.identifier.issue4-
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:Faculty of Veterinary Medicine: Journal Articles
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