DETERMINATION OF BISPHENOL A IN BEVERAGES BY RP-HPLC
Journal
Agriculture & Forestry
Date Issued
2019
Author(s)
Petanovska-Ilievska, Biljana
Jankulovska, S., Mirjana
Velkoska-Markovska, Lenche
Spasovska, Jasmina
DOI
DOI: 10.17707/AgricultForest.65.1.24
Abstract
Bisphenol A (BPA) is a monomer widely used in the production of
polycarbonate, epoxy resins, diacrylates and phenolic resins. A small quantity of
BPA can migrate into the food and thus it can be potential hazard for human
health and environment. Therefore, quantitative determination of BPA is of a
great importance. A fast, simple, precise and economic RP-HPLC method with
UV-DAD detection for quantitative determination of BPA in beverages was
developed. Three different analytical columns were tested: Hypersil ODS (250
mm x 4.6 mm; 5 µm), LiChrospher 60 RP-Select B (125 mm x 4 mm; 5 µm) and
Purospher® STAR RP-18 endcapped (30 mm x 4 mm; 3 µm). Analyzed
beverages were packed in plastic bottles and small glass bottles closed with a
cops coated with epoxy resin on inside. For quantitative determination of BPA
following experimental conditions were established: mobile phase consisted of
acetonitrile/water 50/50 (v/v), flow rate of 1 mL/min, column temperature of 25
oС, injection volume of 5 µL and UV detection at 200 nm. The method was
developed in an isocratic manner and with a reversed phase column. Prior the
analyses the samples were filtrated through syringe filters Spartan – T with pore
size 0.45 µm. The following parameters were determined: retention time,
linearity, limit of detection (LOD), limit of quantification (LOQ), precision,
accuracy, selectivity and sensitivity. The RP-HPLC method with UV-DAD
detection can be successfully used for quantitative determination of BPA in non-
alcoholic beverages without pre-treatment. The BPA was not detected in the
analyzed beverages.
polycarbonate, epoxy resins, diacrylates and phenolic resins. A small quantity of
BPA can migrate into the food and thus it can be potential hazard for human
health and environment. Therefore, quantitative determination of BPA is of a
great importance. A fast, simple, precise and economic RP-HPLC method with
UV-DAD detection for quantitative determination of BPA in beverages was
developed. Three different analytical columns were tested: Hypersil ODS (250
mm x 4.6 mm; 5 µm), LiChrospher 60 RP-Select B (125 mm x 4 mm; 5 µm) and
Purospher® STAR RP-18 endcapped (30 mm x 4 mm; 3 µm). Analyzed
beverages were packed in plastic bottles and small glass bottles closed with a
cops coated with epoxy resin on inside. For quantitative determination of BPA
following experimental conditions were established: mobile phase consisted of
acetonitrile/water 50/50 (v/v), flow rate of 1 mL/min, column temperature of 25
oС, injection volume of 5 µL and UV detection at 200 nm. The method was
developed in an isocratic manner and with a reversed phase column. Prior the
analyses the samples were filtrated through syringe filters Spartan – T with pore
size 0.45 µm. The following parameters were determined: retention time,
linearity, limit of detection (LOD), limit of quantification (LOQ), precision,
accuracy, selectivity and sensitivity. The RP-HPLC method with UV-DAD
detection can be successfully used for quantitative determination of BPA in non-
alcoholic beverages without pre-treatment. The BPA was not detected in the
analyzed beverages.
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