HPLC METHOD FOR DETERMINATION OF ACTIVE INGREDIENTS IN PESTICIDE FORMULATION SWITCH 62,5 WG
Journal
Journal of Agricultural, Food and Environmental Sciences
Date Issued
2021
Author(s)
Velkoska-Markovska, Lenche
Davitkova, Jovana
Jankulovska, Mirjana S.
DOI
10.55302/jafes21752011vm
Abstract
Analytical quality control for pesticides achieved using appropriate methods is essential in
providing their safe and adequate use in agriculture. This study presents a precise, fast, and a
simple analytical method for determining active ingredients cyprodinil and fludioxonil in a
fungicide formulation Switch 62,5 WG using high-performance liquid chromatography
(HPLC). The development of the HPLC method was performed on a ZORBAX Eclipse Plus
C18 (50 x 4.6 mm, 1.8 µm) analytical column using isocratic elution with a mobile phase
consisting of acetonitrile and water in volume ratio 70:30, a flow rate of 1 mL/min, a constant
column temperature at 25 ºC and UV detection at 220 and 270 nm. The run time of analysis
under these chromatographic conditions was about 1.5 min. The method was validated by
testing specificity, linearity, precision, recovery, LOD, LOQ, and accuracy according to the
CIPAC (Collaborative International Pesticides Analytical Council) and SANCO (Directorate
General Health and Consumer Protection) guidelines, and all the tested parameters were found
within acceptance criteria. The values for multiple correlation coefficient (R2 ≥ 0.99), relative
standard deviation (RSD < 1 %), recoveries ranged from 98.95 - 102.26 %, revealed that the
developed method has a good linearity, precision and accuracy. The proposed method is
suitable for routine analysis of active ingredients cyprodinil and fludioxonil in the formulation
Switch 62,5 WG.
providing their safe and adequate use in agriculture. This study presents a precise, fast, and a
simple analytical method for determining active ingredients cyprodinil and fludioxonil in a
fungicide formulation Switch 62,5 WG using high-performance liquid chromatography
(HPLC). The development of the HPLC method was performed on a ZORBAX Eclipse Plus
C18 (50 x 4.6 mm, 1.8 µm) analytical column using isocratic elution with a mobile phase
consisting of acetonitrile and water in volume ratio 70:30, a flow rate of 1 mL/min, a constant
column temperature at 25 ºC and UV detection at 220 and 270 nm. The run time of analysis
under these chromatographic conditions was about 1.5 min. The method was validated by
testing specificity, linearity, precision, recovery, LOD, LOQ, and accuracy according to the
CIPAC (Collaborative International Pesticides Analytical Council) and SANCO (Directorate
General Health and Consumer Protection) guidelines, and all the tested parameters were found
within acceptance criteria. The values for multiple correlation coefficient (R2 ≥ 0.99), relative
standard deviation (RSD < 1 %), recoveries ranged from 98.95 - 102.26 %, revealed that the
developed method has a good linearity, precision and accuracy. The proposed method is
suitable for routine analysis of active ingredients cyprodinil and fludioxonil in the formulation
Switch 62,5 WG.
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