Simultaneous determination of phenmedipham, desmedipham, and ethofumesate in a pesticide formulation by normal-phase high-performance liquid chromatography
Journal
Acta Chromatographica
Date Issued
2008-03
Author(s)
Velkoska-Markovska, L.
Petanovska-Ilievska, B.
Vodeb, L.
DOI
10.1556/achrom.20.2008.1.9
Abstract
A normal-phase high-performance liquid-chromatographic method has been
developed for simultaneous quantitative analysis of phenmedipham, desmedipham, and
ethofumesate in a pesticide formulation. Analysis was performed on a 25 cm × 0.4 cm, 5-
μm particle, CN column with n-hexane–dichloromethane 40:60 (v/v) as mobile phase at
a flow rate of 1 mL min−1. UV detection was performed at 270 nm; the constant column
temperature was 25°C. The run time under these chromatographic conditions was less
than 8 min. Calibration plots were linear in the concentration range 76–380 μg mL−1 for
phenmedipham, 72–360 μg mL−1 for desmedipham, and 52–260 μg mL−1 for ethofume-
sate. Statistical evaluation by analysis of variance showed the intra-day repeatability
(n = 8) and inter-day precision (n = 3) of the assay were satisfactory. The sensitivity of
the method, as the limits of detection (LOD) and quantification (LOQ) for each active in-
gredient, was also determined.
developed for simultaneous quantitative analysis of phenmedipham, desmedipham, and
ethofumesate in a pesticide formulation. Analysis was performed on a 25 cm × 0.4 cm, 5-
μm particle, CN column with n-hexane–dichloromethane 40:60 (v/v) as mobile phase at
a flow rate of 1 mL min−1. UV detection was performed at 270 nm; the constant column
temperature was 25°C. The run time under these chromatographic conditions was less
than 8 min. Calibration plots were linear in the concentration range 76–380 μg mL−1 for
phenmedipham, 72–360 μg mL−1 for desmedipham, and 52–260 μg mL−1 for ethofume-
sate. Statistical evaluation by analysis of variance showed the intra-day repeatability
(n = 8) and inter-day precision (n = 3) of the assay were satisfactory. The sensitivity of
the method, as the limits of detection (LOD) and quantification (LOQ) for each active in-
gredient, was also determined.
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