Size-exclusion HPLC analysis of trace element distributions in hepatic and gill cytosol of Vardar chub (Squalius vardarensis Karaman) from mining impacted rivers in north-eastern Macedonia
Journal
Science of the Total Environment 613–614 (2018) 1055–1068
Date Issued
2018
Author(s)
Nesrete Krasnici, Zrinka Dragun, Marijana Erk, Sheriban Ramani, Maja Jordanova, Katerina Rebok, Vasil Kostov
DOI
https://doi.org/10.1016/j.scitotenv.2017.09.160 0048-9697/©
Abstract
Many bioindicators have not yet been well characterized regarding their tendency to bind trace elements by dif- ferent cytosolic biomolecules in response to trace element exposure. Accordingly, our principal aim was to de!ne the cytosolic distributions of Cd, Co, Cu, Fe, Mn, Mo, Se, and Zn among the biomolecules of different molecular masses in liver and gills of Vardar chub (Squalius vardarensis Karaman), a representative !sh species of Macedo- nian rivers, and to determine distribution changes which occur as a consequence of increased exposure to speci!c trace elements. Additionally, we aimed to con!rm the presence of heat-stable biomolecules in chub hepatic and gill cytosols. Distribution pro!les were obtained by separation of cytosols and heat-treated cytosols using size-ex- clusion high performance-liquid chromatography, and by of"ine determination of trace element concentrations using high resolution inductively coupled plasma-mass spectrometry. Distribution pro!les of trace elements were mainly characterized by several peaks encompassing different ranges of molecular masses, as a sign of in- corporation of trace elements in various biomolecules within hepatic and gill cytosols. Especially interesting !nd- ing was probable binding of Fe to ferritin, which was especially pronounced in the liver, as a sign of important liver function in Fe storage. Furthermore, association with heat-stable proteins, metallothioneins (MT), was indi- cated for Cd, Cu, and Zn in the hepatic cytosol, as well as for Cd in the gill cytosol, whereas a sign of Zn-MT asso- ciation was not observed in the gills. The presence of Mo- and Se-binding heat-stable compounds of very low molecular masses (b10 kDa) in the cytosol was determined for both liver and the gills. Trace elements under all studied conditions were found associated to the same biomolecules, and only their proportions associated to speci!c cytosolic compounds have changed as a consequence of their increased bioaccumulation in the liver and gills of Vardar chub.
Subjects
File(s)![Thumbnail Image]()
Loading...
Name
2018 - Size-exclusion HPLC analysis of trace element distributions in hepatic and gill cytosol of Vardar chub (Squalius vardarensis Karaman) from mining impacted rivers in north-eastern Macedonia.pdf
Size
2.07 MB
Format
Adobe PDF
Checksum
(MD5):3661c5653ecf421f3db92e4672068214