Development of reverse-phase high-performance liquid chromatography method for simultaneous determination of sodium benzoate and potassium sorbate in beverages
Journal
Acta Chromatographica
Date Issued
2017-09
Author(s)
Petanovska-Ilievska, Biljana
Velkoska-Markovska, Lenche
Jankulovska, Mirjana S.
DOI
10.1556/1326.2017.29.3.05
Abstract
Reverse-phase high-performance liquid chromatography (RP-HPLC) method
for simultaneous determination of sodium benzoate and potassium sorbate in beverages
was developed using high speed column. The simple and rapid reverse-phase method
for quantitative determination of both preservatives was established on LiChroCART®
Purospher STAR RP-18e (30 mm × 4 mm; 3 μm) column, mobile phase consisted of ace-
tonitrile–phosphate buffer (pH = 3.5) in volume ratio of 8:92 (v/v), flow rate of 1 mL
min−1, ultraviolet (UV) detection at 195 nm for sodium benzoate and 260 nm for potas-
sium sorbate, and constant column temperature at 25 °C. Linearity, precision, accuracy,
limit of quantification (LOQ), and limit of detection (LOD) were tested for method vali-
dation. Linearity range for sodium benzoate was 6.04–200.27 mg L−1 (R2 = 0.999) while,
for potassium benzoate (R2 = 0.999), 12.19–406.36 mg L−1. The RSD values ≤1.03% dem-
onstrate excellent intra-day precision. LOD for sodium benzoate and potassium sorbate
was 0.004 and 0.003 mg L−1, while LOQ was 0.012 and 0.009 mg L−1, respectively. This
method was applied for quantitative determination of investigated preservatives in bev-
erages which were taken from Macedonian markets.
for simultaneous determination of sodium benzoate and potassium sorbate in beverages
was developed using high speed column. The simple and rapid reverse-phase method
for quantitative determination of both preservatives was established on LiChroCART®
Purospher STAR RP-18e (30 mm × 4 mm; 3 μm) column, mobile phase consisted of ace-
tonitrile–phosphate buffer (pH = 3.5) in volume ratio of 8:92 (v/v), flow rate of 1 mL
min−1, ultraviolet (UV) detection at 195 nm for sodium benzoate and 260 nm for potas-
sium sorbate, and constant column temperature at 25 °C. Linearity, precision, accuracy,
limit of quantification (LOQ), and limit of detection (LOD) were tested for method vali-
dation. Linearity range for sodium benzoate was 6.04–200.27 mg L−1 (R2 = 0.999) while,
for potassium benzoate (R2 = 0.999), 12.19–406.36 mg L−1. The RSD values ≤1.03% dem-
onstrate excellent intra-day precision. LOD for sodium benzoate and potassium sorbate
was 0.004 and 0.003 mg L−1, while LOQ was 0.012 and 0.009 mg L−1, respectively. This
method was applied for quantitative determination of investigated preservatives in bev-
erages which were taken from Macedonian markets.
Subjects
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