Polymorphism of β-lactoglobulin in pramenka breed sheep in Bosnia and Herzegovina
Journal
The Journal of Animal and Plant Sciences
Date Issued
2018
Author(s)
Rustempašić, A.
Dokso, A.
Zečević, E.
Hodžić, A.
Hrković-Porobija, A.
Sarić, Z.
Miskoska–Milevska, E.
Popovski, Z.
Brka, M.
Abstract
The aim of this study was to determinate allele frequency β-lactoglobulin in Pramenka breed sheep in Bosnia and
Herzegovina. The DNA was extracted from blood of 283 sheep, collected from four flocks around Sarajevo, Bosnia and
Herzegovina. The gene β-lactoglobulin was amplified using polymerase chain reaction – restriction fragment length
polymorphism (PCR–RFLP) technique. Two genetic variants (A and B) and three genotypes (AA, AB and BB) of β-
lactoglobulin have been identified. Genotype frequencies of β-lactoglobulin were with the following values: AA (0.325),
AB (0.445), and BB (0.229), while the frequencies of allele A and B were 0.547 and 0.453 respectively. The highest
frequency of genotype AA is present in the location Treskavica (38%), genotype AB in the location Vlašić (52%), while
the genotype BB is mostly present in location Bjelašnica (29%). The population of Pramenka breed sheep was in Hardy-
Weinberg equilibrium. It is expected that in a future the programs in sheep breeding in Bosnia and Herzegovina certainly
would take into account the genetic variants of β-lactoglobulin that could contribute to better production and economic
benefit.
Herzegovina. The DNA was extracted from blood of 283 sheep, collected from four flocks around Sarajevo, Bosnia and
Herzegovina. The gene β-lactoglobulin was amplified using polymerase chain reaction – restriction fragment length
polymorphism (PCR–RFLP) technique. Two genetic variants (A and B) and three genotypes (AA, AB and BB) of β-
lactoglobulin have been identified. Genotype frequencies of β-lactoglobulin were with the following values: AA (0.325),
AB (0.445), and BB (0.229), while the frequencies of allele A and B were 0.547 and 0.453 respectively. The highest
frequency of genotype AA is present in the location Treskavica (38%), genotype AB in the location Vlašić (52%), while
the genotype BB is mostly present in location Bjelašnica (29%). The population of Pramenka breed sheep was in Hardy-
Weinberg equilibrium. It is expected that in a future the programs in sheep breeding in Bosnia and Herzegovina certainly
would take into account the genetic variants of β-lactoglobulin that could contribute to better production and economic
benefit.
Subjects