DETERMINATION OF MALATHION IN PESTICIDE FORMULATION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
Journal
The Journal "Agriculture and Forestry"
Date Issued
2020-12-15
Author(s)
VELKOSKA-MARKOVSKA, Lenche
PETANOVSKA-ILIEVSKA, Biljana
DOI
10.17707/agricultforest.66.4.14
Abstract
A new, simple, fast and reliable high-performance liquid chromatography
(HPLC) method for determination of an active ingredient malathion in the pesticide
formulation has been developed. Successful separation and quantification of
malathion were achieved using Purospher STAR RP-18e (30 x 4 mm, 3 µm) and
isocratic elution with mobile phase consisted of acetonitrile/water (47/53, V/V),
flow rate of 1 mL/min, constant column temperature at 25 °C and UV detection at
220 nm. The specificity, selectivity, linearity, precision, accuracy, limit of
detection (LOD) and quantification (LOQ) were tested for the method validation
according to the CIPAC and SANCO guidelines. The obtained values for multiple
correlation coefficients (R2 > 0.99), relative standard deviation (RSD) of retention
times and peak areas (RSD ≤ 0.64%), and recoveries ranged from 101.04 to 101.84
%, revealed that the developed method has an excellent linearity, precision of
retention time and peak area and accuracy. The proposed method was successfully
applied for determination of an active ingredient malathion in the emulsifiable
concentrate (EC) “Etiol techni” for a run time of 4 min.
(HPLC) method for determination of an active ingredient malathion in the pesticide
formulation has been developed. Successful separation and quantification of
malathion were achieved using Purospher STAR RP-18e (30 x 4 mm, 3 µm) and
isocratic elution with mobile phase consisted of acetonitrile/water (47/53, V/V),
flow rate of 1 mL/min, constant column temperature at 25 °C and UV detection at
220 nm. The specificity, selectivity, linearity, precision, accuracy, limit of
detection (LOD) and quantification (LOQ) were tested for the method validation
according to the CIPAC and SANCO guidelines. The obtained values for multiple
correlation coefficients (R2 > 0.99), relative standard deviation (RSD) of retention
times and peak areas (RSD ≤ 0.64%), and recoveries ranged from 101.04 to 101.84
%, revealed that the developed method has an excellent linearity, precision of
retention time and peak area and accuracy. The proposed method was successfully
applied for determination of an active ingredient malathion in the emulsifiable
concentrate (EC) “Etiol techni” for a run time of 4 min.
Subjects
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