Frequencies of erythrocyte nuclear abnormalities and of leucocytes in the fish Barbus peloponnesius correlate with a pollution gradient in the River Bregalnica (Macedonia)

Katerina Rebok, Maja Jordanova, Valentina Slavevska-Stamenković, Lozenka Ivanova1, Vasil Kostov, Trajče Stafilov & Eduardo Rocha

doi:10.1007/s11356-017-8665-6

Frequencies of erythrocyte nuclear abnormalities and of leucocytes in the fish Barbus peloponnesius correlate with a pollution gradient in the River Bregalnica (Macedonia)

Journal

Environ Sci Pollut Res (2017) 24:10493–10509

Date Issued

2017

Author(s)

Katerina Rebok, Maja Jordanova, Valentina Slavevska-Stamenković, Lozenka Ivanova1, Vasil Kostov, Trajče Stafilov & Eduardo Rocha

DOI

10.1007/s11356-017-8665-6

Abstract

Integrated chemical and biomarker approaches were performed to estimate if there is ongoing toxicity in the River Bregalnica, namely connected with the presence of metals. The study was performed in water, sediment, and bar- bel (Barbus peloponnesius), collected in two seasons, from two suspected polluted and one reference zones. The water analyses revealed higher mean values in polluted sites for most of the examined physicochemical parameters. Metal concentrations (Zn, Cu, Cd, Mn, Pb, and Fe) in water were more or less constant, whereas in sediment, they were higher at the two polluted locations. Condition factor (CF), as a gen- eral health indicator, revealed better overall condition in bar- bel from the reference site. In general, blood parameters re- vealed higher values in the polluted localities. Irrespective of sex and/or season, the frequency of micronuclei (MN) and vacuolated nuclei (VN) were with higher rates in polluted sites. Similarly, the frequencies of the leucocytes (Le), binuclei (BN), and irregularly shaped nuclei (ISN) were also significantly increased in the polluted localities, but they seemed prone to be influenced by sex and/or season. However, strong positive correlations between blood bio- markers and most water physicochemical parameters and met- al in sediment were estimated. Our data support that the River Bregalnica’s lower course receives significant genotoxic pol- lution, likely via metal industry effluents, agricultural runoff, and domestic sewage, and reinforced the utility of MN and other nuclear abnormalities as sensitive and suitable bio- markers for genotoxicity when used in monitoring studies

Subjects

Blood . Fish . Genoto...

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