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    LABORATORY TESTS IN MONITORING HEALTH STATUS IN SHEEP FLOCKS
    (Serbian veterinary society, 2017-09)
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    Animal Stress as a Risk Factor in Animal Rescuing Operations
    (Faculty of Veterinary Medicine-Skopje, Ss. Cyril and Methodius University in Skopje, North Macedonia, University of Ljubljana, Veterinary Faculty and Harran University, 2017-07-08)
    Martin Nikolovski
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    Miroslav Radeski
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    Dean Jankuloski
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    The Dynamics of Biochemical Parameters in Blood of Clinically Healthy Holstein Cows from Day 5 before to Day 60 after Calving
    (Faculty of Veterinary Medicine - Skopje, 2015-10-01)
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    Janevski, Aleksandar
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    Kirovski, Danijela
    <jats:title>Abstract</jats:title> <jats:p> The peripartal period in Holstein dairy cows is critical, due to the transition from pregnancy to lactation. We have studied the dynamics of biochemical parameters from day 5 before to day 60 after calving. The study included 10 multiparous Holstein cows, examined at days -5, 5, 10, 30 and 60 relative to calving. Blood samples were taken from vena jugularis. Analyzed biochemical parameters were glucose, triglycerides, total cholesterol, total bilirubin, albumin, total protein, urea, NEFA and BHBA. Milk production and body condition score were also estimated. Obtained results showed that cows were exposed to mild to marked metabolic distress. Energy status was changed due to increased values of NEFA and BHBA and decreased value of glucose after calving. Protein concentrations were increased at day 10 after calving, despite the decrease of the level of albumin. Urea concentrations before and after calving were within physiological range indicating an optimal protein diet. Increased values of total bilirubin at day 5 after calving indicated liver increased activity. Lipid status presented by triglycerides and total cholesterol revealed no differences in blood concentrations. Milk production was highest at day 30 after calving. BCS were highest in dry cows, thereafter they declined and recovered at day 60 after calving.</jats:p> <jats:p>In conclusion, biochemical parameters can be used as relevant indicators of metabolic distress in cows around calving with milk and BCS recording as aside parameters. Changes in some biochemical parameters indicate liver increased activity and metabolic stress, that could lead to decreased milk production, impaired reproductive performance and, finally, to illness.</jats:p>
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    Influence of the Season on the Metabolic Profile in Chios Sheep
    (Faculty of Veterinary Medicine - Skopje, 2015-10-01)
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    Janevski, Aleksandar
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    Kirovski, Danijela
    <jats:title>Abstract</jats:title> <jats:p> Chios is a breed of sheep selected for milk production, with metabolic features typical for a dairy sheep breed. The energy requirements of pregnant sheep is increase in the last weeks of gestation. Metabolic imbalance in the late pregnancy in sheep, usually cause a metabolic disorder known as pregnancy toxemia. Additionally, a pregnant sheep exposed to low environmental temperatures has increased energy demands, due to its adaptation to undesirable environmental conditions. The aim of this study was to compare the metabolic profile of Chios sheep exposed to different environmental conditions.Two groups of ewes were instigated. First group included 8 pregnant ewes with clinical signs of pregnancy toxemia exposed to cold stress during the winter season. The second group included 8 non-pregnant, clinically healthy ewes, that were examined during the non-breeding period, in the spring season. Blood samples were taken and serum concentrations of glucose, beta-hydroxybutirate (BHBA), total protein, albumin, urea, creatinine, triglyceride and cholesterol, as well as activity of AST and ALP were determined. Pregnant ewes exposed to cold stress had significantly lower levels of glucose and total protein, and significantly higher levels of BHBA, albumin and AST in the serum compared to non-pregnant ewes that were in optimal environmental conditions. There was no significant difference between the serum levels of urea, creatinine, cholesterol, triglycerides and ALP among the groups. In conclusion, low environmental temperature and poor feeding during the winter season caused metabolic distress in pregnant ewes during the early winter season.</jats:p>