Blagoevska, Katerina
Preferred name
Blagoevska, Katerina
Official Name
Blagoevska, Katerina
Main Affiliation
Email
kblagoevska@fvm.ukim.edu.mk
22 results
Now showing 1 - 10 of 22
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Item type:Publication, Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis(MDPI AG, 2020-06-29); - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Effect of t-2 mycotoxin on glut-2 expression in the broiler's gastrointestinal tract(University of Tartu, 2016-12-21); Fusarium spp. fungus which is toxic to humans and animals. Deleterious effects of these feed contaminants in animals are well documented, ranging from growth impairment, decreased resistance to pathogens, hepato- and nephrotoxicity to death. As the initial interaction of mycotoxins is with the gut epithelium the epithelium is repeatedly exposed to these toxins at higher concentrations than other tissues. Several methods for the determination of T-2 toxin based on traditional chromatographic, immunoassay, or mass spectroscopy techniques have been described. As up to now there is lack of knowledges about the effect of T-2 mycotoxin on the expression of glucose transporters in the proximal part of the gastrointestinal tract (GIT), the aim of our investigation was to study the experssion of glucose transporter-2 (GLUT-2) in the epithelial cells of glandular stomach and duodenal mucosa comparatively in normal and T-2 toxicated broilers. Material from the two parts of GIT was collected from six 7-day-old normal female broilers and six 7-day-old female broilers with T-2 toxicosis. The specimen were fixed with 10% formalin, embedded into paraffin, slices 7 μm thick were cut followed by immunohistochemical staining with polyclonal primary antibody Rabbit anti-GLUT-2 carried out according to the manufacturers quidelines (IHC kit, Abcam, UK). The results showed the moderate expression of GLUT-2 in epithelial cells both in the glandular as well as in the duodenal mucosa in the control group’s chicken and the weaker expression of GLUT-2 after T-2 toxin administration indicating to the reduced glucose transport in the gastrointestinal system during T-2 mycotoxicosis.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Determination of Aflatoxins and Ochratoxin a in Animal Liver Using HPLC-FD Method with Immunoaffinity Column Clean-Up(Walter de Gruyter GmbH, 2021-05-12) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Radiation levels in samples of dicalcium phosphate (DCP) with a gamma spectrometry method.(UNIVERSITY OF EAST SARAJEVO FACULTY OF AGRICULTURE, Republic of Srpska, B&H et al, 2021-10-07); ; ; Stojanovska-Dimzoska, Biljana - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Radiocesium concentration in mushrooms collected in the Republic of North Macedonia(Agricultural University of Tirana, 2021-11-03); ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Measurement of the 226Ra, 232Th and 40K activities in corn in the Republic of North Macedonia and resulting annual radiation dose by ingestion(University of Banja Luka Faculty of Agriculture University City Bulevar vojvode Petra Bojovića 1A 78000 Banja Luka, Republic of Srpska, B&H, 2021-05-27); ; ; Considering the fact that there has been a significant population growth and this increases the need of food, various agricultural activities are increasingly being used, including the use of phosphate fertilizers that can directly affect agricultural crops. Therefore, it is necessary to know the level of present radionuclides, especially in corn, which is very often used for the needs of people and animals. The purpose of this study was focused on comparing the activity concentrations of 226Ra, 232Th, 40K in corn samples, and the calculated results were the basis for the assessment of the external hazard index (Hex), the internal hazard index (Hin), the radium equivalent activity (Raeq), and the annual gonadal dose equivalent (Dgon). The gamma spectrometry technique was used for measurement of the samples, i.e. an instrument - gamma spectrometer (Canbera Packard) with a high purity germanium detector. The obtained spectra from the measurement were analyzed by using the GENIE 2000 program. Based on the performed examinations, the activity concentrations in corn ranged from 0.42 to 0.77 Bq kg - 1 for 226Ra, from 0.06 to 0.87 Bq kg - 1 for 232Th, and from 117.54 to 322.11 Bq kg - 1 for 40K. The mean value of the radiation hazard index, the Heks value is lower than the maximum allowed value which is <1 for Heks. The value of the radium equivalent activity Raeq ranges from 9.01 to 23.16 and is below the maximum recommended limit, i.e. 370 Bq kg-1. The annual gonadal dose equivalent has an average value of 75.62. The research in this study shows that all samples of corn have no biological hazard i.e. it was found that the natural levels of radioactivity are not within the high-risk range and are below the international standards. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Detection of Enterotoxigenic Potential of <i>Staphylococcus Aureus</i> Isolates from Cheese Samples with Two Different Methods(Macedonian Veterinary Review, 2022-03-29); ; <jats:title>Abstract</jats:title> <jats:p>The primary objective of our study was to detect the occurrence of enterotoxigenic <jats:italic>Staphylococcus aureus</jats:italic> in diverse types of cheese (cow’s milk cheese and mixed milk cheese) samples from R.N. Macedonia. Cheese samples were analyzed for enumeration and isolation of the <jats:italic>S. aureus</jats:italic> strains according to ISO 6888-1. We detected the toxigenic potential of the strains by the use of the Enzyme Link Fluorescent Assay VIDAS system, and we confirmed the presence of the SEs (<jats:italic>sea, seb, sec, sed, see</jats:italic>) genes by multiplex PCR. The results showed that out of 270 samples of cheese, coagulase-positive staphylococci (CPS) were detected in 27 (10%), and coagulase-negative staphylococci in five samples (1.8%). Biochemically, all 27 CPS samples were confirmed to be <jats:italic>Staphylococcus aureus.</jats:italic> With VIDAS SET2 test we confirmed that 11 isolates are producers of one of the toxins limited by the test. With the conventional PCR we confirmed genes in only 7 isolates. Most common detected gene was <jats:italic>seb</jats:italic> n=3 (42.8%), followed by <jats:italic>sea</jats:italic> n=2 (28.6%), and <jats:italic>sec</jats:italic> n=2 (28.6%). Additionally, <jats:italic>sed</jats:italic> and <jats:italic>see</jats:italic> genes were not detected in any of the <jats:italic>S. aureus</jats:italic> isolates. Discrepancies between the two test methods for detection of enterotoxigenic potential are not uncommon. The presence of viable <jats:italic>Staphylococcus aureus</jats:italic> cells that have enterotoxin potency demonstrates the importance of appropriate hygiene practices in the diary process and also the maintenance of the products in order to obtain a safe final product for the consumers.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Determination of heavy metals and radionuclides in animal feeds of plant origin.(Agricultural University of Tirana, 2021-11-03); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, RADIOACTIVITY LEVELS IN SOME MUSHROOM SPECIES COLLECTED FROM MACEDONIA AND CONSEQUENT DOSES(University of East Sarajevo, Faculty of Agriculture, 2022); ; Stojanovska-Dimzoska, BiljanaMushrooms are bioindicators in the environment that accumulate radionuclides, therefore they can pose a radiological threat. In the Republic of Macedonia, mushrooms are widely consumed as a part of the diet, for this reason their radiological control is required. In this research, the activity concentrations in different types of mushrooms (Boletus edulis, Marasmius oreades, Morchela conica, Lactarius deliciosus) were determined. The mushrooms were collected from six different locations in the Republic of Macedonia, and information on the radiation dose for the general population were obtained with this measurement. The analyses were performed by means of HPGe gamma spectrometry, i.e. an instrument - gamma spectrometer (Canbera Packard) with a high purity germanium detector. The obtained spectra from the measurement were analyzed by using the GENIE 2000 program. On the basis of the performed tests, the mean values for the activity concentrations in the mushrooms were as follows 41.9±1.2 Bq kg − 1 for 226Ra, 40.3±1.8 Bq kg − 1 for 232Th, 83.75±6.2 Bq kg − 1 for 40K, and 2.34±0.24 Bq kg − 1 for 137Сs. The mean value of the radiation risk index Heks is lower than the maximum allowed value which is <1 for Heks. The value of the radium equivalent activity Raeq is below the maximum recommended limit, i.e. 370 Bq kg-1. Significant variations were not observed in regard to the activity concentrations of 137Cs and 40K in the same mushroom species from different sampling points. High activity concentrations of 137Cs activity were not detected in any of the mushrooms. The researches in this study show that all mushroom samples pose no biological threat, i.e. it was found that the analyzed radionuclides do not pose a health risk and the levels are below the international standards. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, EVALUATION OF THE ANALYTICAL VALUE OF SARS-CoV-2 ANTIGEN TEST IN RELATION TO Ct-VALUES OF RT-qPCR IN PATIENTS SUSPECTED OF COVID-19(Македонско лекарско друштво = Macedonian Medical Association, 2021); ; Introduction. COVID-19 pandemic threatens global human health. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is a reference test for identification of acute SARS-CoV-2 infection, but it is associated with results delay. There is a need of fast and reliable tests which can improve the efforts of controlling the transmission of SARS-CoV-2. Aim. The aim of this study was to determine the analytical value of the rapid SARS-CoV-2 Ag-test in relation to the Ct values of the RT-qPCR. Methods. The study group comprised outpatients suspected for COVID-19, sampled twice, first for the routine RT-qPCR, and second for SARS-CoV-2 antigen testing. The results obtained by the rapid antigen test (Panbio™ COVID-19) were evaluated in relation to Ct values of the SARS-CoV-2 E-gene, obtained by RTqPCR Allplex 19-nCoV multiplex assay platform. Results. SARS-CoV-2 prevalence, based on RT-qPCR, was 50.8% (186/366). Specificity of the PanbioTM COVID- 19 Ag Rapid Test was 100%. Test sensitivity was 73.8%. Restricting RT-qPCR to Ct-values<30 increased test sensitivity to 91.2%. Conclusion. The findings underscored the epidemiological value of the rapid Ag-test since it reliably identifies contagious SARS-CoV-2 infected individuals who actively spread the virus in the community.
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