Kotevska, Vesna

Preferred name

Kotevska, Vesna

Official Name

Kotevska, Vesna

Main Affiliation

vesna.kotevska@medf.ukim.edu.mk

Now showing 1 - 10 of 21

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COMPARISON OF THE SUSCEPTIBILITY OF ESBL-POSITIVE AND ESBL-NEGATIVE ISOLATES OF E. COLI AND KLEBSIELLA PNEUMONIAE TO ANTIMICROBIAL AGENTS
(Macedonian Association of Anatomists and Morphologists (MAAM), 2014)
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The aim of this investigation was to compare the susceptibility between ESBL-positive and ESBL-negative isolates to different antimicrobial agents. Material and methods: A total of 1207 consecutive non-repeat isolates of E. coli and K. pneumoniae-Kp (E. coli-804, Kp-403) were obtained from different clinical specimens from patients hospitalized at the University Clinics in Skopje over a one year period. Conventional microbiological procedures for the isolation and detection of the strains were used. Susceptibility to beta-lactams and non-beta-lactams was determined by standard disk diffusion method and by automated method using Vitek (for determination of the MIC-minimal inhibitory concentration). A total of 251 isolates were selected (E. coli-126 and K. pneumoniae-125) using tests for phenotypic detection of the ESBL-production. A total of 233 randomly selected strains which were ESBL-negative by phenotypic tests (E.coli-130 and K.pneumoniae103) were used as a control strains for susceptibility testing. Results: Difference of the susceptibility to imipenem between ESBL-positive and ESBL-negative strains of E. coli and K. pneumoniae was not detected. Both groups of strains were 100% susceptible to imipenem. Considering the rest of the tested beta-lactams, as well as non-beta lactams and uroantiseptics, there was a difference in the susceptibility comparing both groups of strains of E. coli and K. pneumoniae. This was due to the fact that ESBL-negative strains were highly susceptible to all tested antimicrobial agents, unlike the ESBL-positive ones (except in cases of cotrimoxazole and nitrofurantoin, where the difference in susceptibility between ESBL-positive and ESBL-negative strains of E. coli was not statistically significant, because both groups of strains were susceptible to those antimicrobial agents).These data are important for recommending adequate treatment of infections with ESBL-producing bacteria.
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Microbiological analysis of wound samples
(Medical Faculty, Ss. Cyril and Methodius University in Skopje, 2020)
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Cvetanovska S
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Етиологија на инфекциите на долните дишни патишта во детската возраст и значењето на директниот микроскопски препарат во нивната дијагностика
(Македонско лекарско друштво = Macedonian Medical Association, 2003)
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Spasenovski, Trajce
Цел на испитувањето е да е направи анализа на етиологијата на инфекции на долните дишни патишта (ДДП) кај деца според две различни микробиолошки испитувања: културелно и серолошко; да се компарираат микробиолошките наоди според етиолошкиот агенс (бактериски, вирусни, мешани) со наодите во директиот микроскопски препарат. Беа испитани трахеални аспирати (ТА) и крв од вкупно 118 деца со инфекции на ДДП од Клиниката за детски болести (ноември 2001-март 2002). ТА се испитуваа културелно и микроскопски (епителни клетки и леукоцити). Наодите беа групирани во 3 микроскопски категории: I (>25 ЛЕ, висока гнојност, <10 еп. кл. незначителна контаминација); II (>25 еп. кл. значителна контаминација; <10 ЛЕ ниска гнојност) и III (<10 ЛЕ, ниска гнојност, , <10 еп. кл. незначителна контаминација). Во серумот со Pneumo-slide тест се одредуваа ИгМ антитела на 4 атипични бактерии и 5 вируси. Со позитивен микробиолошки наод беа 109 деца. Докажани беа 243 микроорганизми (58 култивабилни бактерии, 29 атипични бактерии и 156 вируси) со најчеста застапеност на: S. pneumonia (29), M pneumonia (21), H influenzaе (17), C. burneti (7), ентеробактерии (8), Инфлуенца вирус Б (82) и Инфлуенца вирус А (74). Дваесет (18,3%) деца имаа бактериска инфекција, 38 (34,8%) вирусна а 51 (46,7%) мешана инфекција (бактериско/вирусна). Според директните микроскопски препарати, 46 (38,9%) ТА беа во I микроскопска категорија, 31 (26,2%) во II и 41 (34,7%) во III. Микроскопското испитување покажа дека бројот на децата со вирусни инфекции е поголем, 64 (58,7%) од оние со мешани 25 (23,0%). Бактериолошката (културелна+директен микроскопски препарат) и серолошката дијагностика е значајна за детекција на вистински етиолошки агенси на инфекции на ДДП (бактериски, вирусни), за одредување квалитет на примероците за обработка, за примена на соодветна антимикробна терапија.
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Pneumococcal serotypes and their resistance to penicillin
(Faculty of Medicine, Ss. Cyril and Methodius University, Skopje, R Macedonia, 2014)
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Testicular infection in brucelosis: Report of 34 cases
(Elsevier, 2018-02)
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Silvana Miskova
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Danco Balalovski
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Background/Purpose To present clinical and laboratory features, treatment options, and outcome in patients with brucellar testicular infection and to compare them with analogous in brucellar patients without testicular involvement. Methods Thirty four brucellar patients with testicular infection treated in two general hospitals in the Republic of Macedonia, during the period 1998–2009, were retrospectively analyzed. Their clinical and laboratory characteristics were compared with analogous in 364 male brucellar patients without testicular infection, who were treated at the same hospitals during the same time period. Results Brucellar testicular infection was evident in 34 (8.5%) out of 398 male patients with brucellosis. The median age of the patients was 46.5 years. In all patients testicular involvement was presented as an acute form with a median duration of 5 days (range, 2–14 days) prior to diagnosis. Twenty-three of the patients had at least one other simultaneous focal infection. After starting with the treatment testicular infection lasted a median 10 days, range 7–21 days. Brucellar patients with testicular infection when compared with other brucellar patients more frequently manifested fever (97% vs. 61%), concomitant spondylitis (32% vs. 16%), and urinary system involvement (12% vs. 2%). Also, the relapse rate in patients with testicular involvement was significantly higher (24% vs. 9%). Conclusion In endemic regions brucellosis should be taken into consideration in any patient with testicular infection. Brucellar testicular involvement is usually characterized with a severe acute clinical presentation and a high percentage of relapses which entails the need of timely recognition and proper treatment duration of at least 60 days.
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Phenotypic and genetic relationship of Acinetobacter Baumannii isolates
(Македонска академија на науките и уметностите, Одделение за биолошки и медицински науки = Macedonian Academy of Sciences and Arts, Section of Biological and Medical Sciences, 2011)
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G. Jankoska
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The interest in Acinetobacter continues to rise. One of the main reasons is the emergence of multi-resistant strains, which cause outbreaks of infection involving several patients in a ward, in the intensive care unit and in different areas of the hospital. Many outbreaks of its infection or colonization in surgical, neonatal and burn intensive care units have been reported, but the epidemiology of these infections remains unclear. Aim: To investigate the relationship among the isolates of Acinetobacter baumannii, comparing some of their phenotypic and genetic features. Material and methods: A total of 20 Acinetobacter baumanni isolates were included in the study. 12 strains of Acinetobacter baumannii were obtained within a week in July 2010, from neonates hospitalized at the paediatric intensive care unit and on the neonatal ward. Three strains were isolated from neonates at the paediatric intensive care unit three months ago. All the Acinetobacter baumannii strains were isolated from tracheal aspirates obtained from neonates with infection of the lower respiratory tract. Five additional Acinetobacter baumannii strains were included in the study as controls. They were isolated from wound swabs taken from adult patients with wound infection, hospitalized at the University Traumatology Clinic. Susceptibility of the bacterial strains to 13 different antimicrobial agents was determined by the disk diffusion method (Kirby-Bauer). Additional testing of the susceptibility was performed by the VITEK 2 system. RAPD-PCR fingerprinting was carried out using the following primer (5' GAAACAGCTATGACCATG -3'). Results: All A. baumannii isolates were multi-drug resistant. Antibiotic susceptibility-testing by the disk-diffusion method and automated VITEK 2 system showed 3 and 2 antimicrobial susceptibility patterns, respectively. RAPD-PCR assay of A. baumannii strains revealed two different RAPD-fingerprints. All the strains of A. baumannii isolated within a week in July 2010 from tracheal aspirates taken from neonates in the paediatric intensive care unit and neonates in the paediatric ward revealed the same RAPD-fingerprint, as well as 3 strains of A. baumannii isolated from tracheal aspirates taken from neonates in the paediatric intensive care unit three months ago. 5 strains of A. baumannii isolated from wound swabs of patients hospitalized at the Traumatology Clinic revealed a different RAPD-fingerprint. Conclusion: All the strains of A. baumannii isolated from neonates in the paediatric intensive care unit and paediatric ward were multi-drug resistant. Investigating the resistance patterns in multi-resistant isolates of Acinetobacter is a useful method which can predict the strain relationship. This method could be completed by at least one molecular method, such as the RAPD-PCR technique, which has shown itself to be a convenient and more reliable in interpreting the strain relationship of the A. baumannii isolates. Good infection control procedures, including phenotypic and molecular typing of A. baumannii isolates, are essential for preventing outbreaks of multi-drug resistant A. baumannii infections in our hospitals.
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Multidrug resistant infections in intensive care units
(Association of medical doctors "Sanamed" Novi Pazar, 2020)
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Background: Intensive care units (ICU) are often the epicentre of development of infections caused by multidrug resistant (MDR) organisms. Purpose: The aim of our study was to determine the prevalence and types of ICU-acquired infections, pathogens associated with such infections and to determine the antibiotic resistance pattern of the presented pathogens. Material and methods: In the study were included 130 patients hospitalized into the surgical ICU of the University Clinic for Anesthesiology and Intensive Care in Skopje in period of 2 months, April -Jun, 2017. who developed infection after at least 72 hours of their hospitalization. In all of them the pathogens and their antibiotic resistance pattern were identified. Results: Twenty of 130 (15.4%) patients developed ICU-acquired infection. Most common infections were pneumonia (50%) and surgical site (30%) infections.Gram-negative organisms were more common isolated than Gram-positive organisms (83% vs.17%). The most common isolated bacteria were Acinetobacter species (30, 41.7%) and Pseudomonas aeruginosa (15, 20.8%). All isolated species were MDR organisms resistant to the most used antibiotics like Cephalosporins, Gentamicin, Ciprofloxacin and Clindamycin. Pseudomonas aeruginosa and Acinetobacter species were sensitive to Colistin, Methicillin-resistant Staphylococcus aureus (MRSA) to Vancomycin and Linezolid and Enterococcus only to Linezolid. Klebsiella pneumoniae and Proteus mirabilis showed low resistance only to Amikacin and Carbapenems. Conclusions: Our study obtained local data about the prevalence and types of ICU-acquired infections, types of pathogens and their antibiotic resistance pattern.Based on this knowledge, clinicians can choose appropriate antibiotics, avoiding antibacterial drug overuse and MDR bacteria development.
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Streptococcus agalactiae во женскиот генитален тракт
(Македонско лекарско друштво = Macedonian Medical Association, 2002)
Јанкоска Гордана
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Спасеновски Трајко
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Phenotipic and genetic relationship of Acinetobacter baumannii isolates
(Macedonian Academy of Sciences and Arts, 2011)
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Jankoska, G
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Mircevska, Gordana
The interest in Acinetobacter continues to rise. One of the main reasons is the emergence of multi-resistant strains, which cause outbreaks of infection involving several patients in a ward, in the intensive care unit and in different areas of the hospital. Many outbreaks of its infection or colonization in surgical, neonatal and burn intensive care units have been reported, but the epidemiology of these infections remains unclear. Aim: To investigate the relationship among the isolates of Acinetobacter baumannii, comparing some of their phenotypic and genetic features. Material and methods: A total of 20 Acinetobacter baumanni isolates were included in the study. 12 strains of Acinetobacter baumannii were obtained within a week in July 2010, from neonates hospitalized at the paediatric intensive care unit and on the neonatal ward. Three strains were isolated from neonates at the paediatric intensive care unit three months ago. All the Acinetobacter baumannii strains were isolated from tracheal aspirates obtained from neonates with infection of the lower respiratory tract. Five additional Acinetobacter baumannii strains were included in the study as controls. They were isolated from wound swabs taken from adult patients with wound infection, hospitalized at the University Traumatology Clinic. Susceptibility of the bacterial strains to 13 different antimicrobial agents was determined by the disk diffusion method (Kirby-Bauer). Additional testing of the susceptibility was performed by the VITEK 2 system. RAPD-PCR fingerprinting was carried out using the following primer (5' GAAACAGCTATGACCATG -3'). Results: All A. baumannii isolates were multi-drug resistant. Antibiotic susceptibility-testing by the disk-diffusion method and automated VITEK 2 system showed 3 and 2 antimicrobial susceptibility patterns, respectively. RAPD-PCR assay of A. baumannii strains revealed two different RAPD-fingerprints. All the strains of A. baumannii isolated within a week in July 2010 from tracheal aspirates taken from neonates in the paediatric intensive care unit and neonates in the paediatric ward revealed the same RAPD-fingerprint, as well as 3 strains of A. baumannii isolated from tracheal aspirates taken from neonates in the paediatric intensive care unit three months ago. 5 strains of A. baumannii isolated from wound swabs of patients hospitalized at the Traumatology Clinic revealed a different RAPD-fingerprint. Conclusion: All the strains of A. baumannii isolated from neonates in the paediatric intensive care unit and paediatric ward were multi-drug resistant. Investigating the resistance patterns in multi-resistant isolates of Acinetobacter is a useful method which can predict the strain relationship. This method could be completed by at least one molecular method, such as the RAPD-PCR technique, which has shown itself to be a convenient and more reliable in interpreting the strain relationship of the A. baumannii isolates. Good infection control procedures, including phenotypic and molecular typing of A. baumannii isolates, are essential for preventing outbreaks of multi-drug resistant A. baumannii infections in our hospitals.
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Clinical characteristics of human brucellosis in patients with various monoarticular involvements
(Springer Science and Business Media LLC, 2016-10)
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Zezoski, Marjan
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Siskova, Dijana
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Miskova, Silvana
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The aim of the study was to determine the main demographic, epidemiological, clinical characteristics, and outcome in patients with various types of brucellar monoarticular involvement. Retrospectively, we analyzed medical histories of 331 patients with brucellar monoarticular involvement who were treated at the infectious diseases departments in Prilep, Shtip, and Veles, Republic of Macedonia, during the period 1990-2012. Their data were compared accordingly to the affected joint (sacroiliac, hip, knee, ankle, wrist, and shoulder).Patients with shoulder arthritis were significantly the oldest (mean ± standard deviation [SD] 46.0 ± 14.5 years) whereas sacroiliitis and hip arthritis were present predominantly in younger patients (mean ± SD 28.7 ± 14.1 and 28.3 ± 18.3 years, respectively) (p = 0.014). Shoulder arthritis duration was significantly the longest (mean ± SD 24.5 ± 12.4 days), and wrist arthritis duration was significantly the shortest (mean ± SD 4.1 ± 2.5 days) (p < 0.001), before establishing the diagnosis of brucellosis. With appropriate treatment, the need for restitution of the joint impairment was significantly longer when sacroiliitis and hip arthritis were present (mean ± SD 32.8 ± 23.0 and 24.6 ± 12.5 days, respectively) (p < 0.001). The relapses were noted in 14.5, 14, 16.5, 5.5, 6, and 5.5 % of the patients with sacroiliitis, hip-, shoulder-, knee-, ankle-. and wrist arthritis, respectively. In endemic areas, brucellosis should be included in the differential diagnostic consideration in patients with monoarticular involvement. Knee-, ankle-, and wrist arthritis seem to be more benign and with appropriate treatment result in short duration and satisfactory outcome. On the other hand, the involvement of sacroiliac, hip-, and shoulder joint deserves more serious approach due to longer arthritis duration and higher frequency of relapses.

Kotevska, Vesna

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