Jovchevski, Radomir
Preferred name
Jovchevski, Radomir
Official Name
Jovchevski, Radomir
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Item type:Publication, Detection of Biofilm Production and Antimicrobial Susceptibility in Clinical Isolates of Acinetobacter baumannii and Pseudomonas aeruginosa(Институт за јавно здравје на Република Македонија = Institute of public health of Republic of Macedonia, 2022-08); ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Antimicrobial resistance in Gram-positive bacteria isolated from blood culture(Macedonian Association of Anatomists and Morphologists, 2024-10); ; ; ; Blood cultures are essential in diagnosing the existence of bacteria in the blood and they continue to be the gold standard for the diagnosis of septicemia. From Gram-positive bacteria, Staphylococcus aureus and Enterococcus spp. are the most common bacteria causing sepsis and septic shock. They are the leading causes of hospital-acquired infections globally. Aim of the paper was to identify Staphylococcus aureus and Enterococcus spp. from hemoculture and to determine their susceptibility to antibiotics. Blood samples from hospitalized patients admitted for routine laboratory testing at the Institute of Microbiology from January 2023 to December 2023 were included in the study. Blood cultures bottles were incubated in automated Bact/Alert system. Isolated colonies were identified using automated VITEK 2 Compact system. MSSA and MRSA were isolated in 48.2% and 51.8% respectively. All isolates 100% were resistant to penicillin. Intermediate sensitivity to levofloxacin 92%, 60% and ciprofloxacin 96%, 64% was observed in MSSA and MRSA strains. Resistance to gentamicin of 63% was seen in MSSA isolates. Non-VRE and VRE were isolated in 67% and 33% respectively. Non-VRE strains had higher resistance towards gentamicin 77%, streptomycin 66%. VRE strains were resistant to gentamicin 93%, ampicillin 85%. Our findings give analysis of Gram-positive bacteria isolated from hemoculture and their resistance pattern. The findings give clinicians an insight for the right treatment option. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Seminal Fluid Microbiota and Male Infertility: An Emerging Frontier(Macedonian Association of Anatomists and Morphologists, 2024-10); ; ; ; Infertility is a multifaceted issue affecting millions globally, with male factors contributing to approximately 50% of cases. Traditionally, male infertility has been linked to genetic, hormonal, and environmental factors. However, emerging research highlights that disruptions or imbalances of the microbiota residing in the male reproductive tract, including the prostate, seminal vesicles, and urethra have significant implications for male reproductive health. Male urogenital system, particularly the urethra is home to a wide variety of microorganisms with rather high quantity. The composition of male genital microbiome can vary significantly between individuals and while the core microbiome is consised of a few dominant species a high variability is noted in minor species. It is predominantly composed of bacteria, with species from the genera Lactobacillus, Staphylococcus, Corynebacterium, and Propionibacterium being commonly found. Differentiating between pathogenic bacteria and the usual resident microflora is a difficulty in clinical practice, especially when it comes to male infertility. Furthermore, even though it is often benign, an imbalance in the commensal microbiota might encourage inflammation or foster an atmosphere that is favorable for pathogenic infections. Therefore, it is crucial to comprehend and maintain this equilibrium in order to create successful plans for the treatment and prevention of infertility. Traditional diagnostic approaches on male infertility focus on hormonal levels, sperm analysis, sperm culture and genetic factors, but unlike conventional culture methods which might find it difficult to distinguish between pathogenic and benign microorganisms because of genotypic and phenotypic overlaps and low pathogen presentation, developments in molecular methods for microbiota analysis, offer the possibility of more precise diagnosis and treatment of urogenital infections and imbalances. Modulating the genital microbiome could offer new treatment strategies for male infertility and related conditions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, THE ROLE OF THE FAECAL SAMPLES CULTIVATION IN THE DIAGNOSTICS OF CLOSTRIDIOIDES DIFFICILE INFECTION(University Ss. Cyril and Methodius in Skopje, 2025-11-25); ; ; ; <jats:p>Introduction: Clostridioides difficile is one of the most important intra-hospital pathogens. A few years ago, a new diagnostic algorithm for Clostridioides difficile infection (CDI) was introduced by ESCMID, stating that cultivation of the bacteria is not necessary for the diagnosis of CDI. Aim: We aimed to explore the role of the cultivation of faecal samples in the diagnosis of CDI. Materials and Methods: In 4 years, we have cultivated 80 different strains of C. difficile from as many patients. Initially, 1380 faecal samples from CDI-suspected patients were planted on selective and non-selective blood agar media and were accordingly incubated in order to isolate the strains. Quick immuno-chromatographic tests were performed on each faecal sample for detection of GDH (glutamate dehydrogenase) and C. difficile toxins A and B as well as on each confirmed C. difficile isolate (from one colony-toxins detection only). Results: In 14 of the 80 faecal samples from which the isolates originated, toxins A and B have not been detected. In 8 of these 14 strains, toxins A and B were detected only from the culture. Only 6 strains were confirmed as non-toxigenic. Conclusions: Although there was no statistical significance, the detection rate of C. difficile toxins A and B was higher from the culture than from the faecal samples by up to 10%. Cultivation of the samples for C. difficile could reduce the rate of false negative findings for CDI.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Successful treatment of pan-drug resistant Acinetobacter baumannii meningitis/ventriculitis following craniotomy and external ventricular drainage: a case report(Oxford University Press and JSCR Publishing Ltd., 2024-09); ; Healthcare-associated central nervous system infections are a significant complication for patients undergoing neurosurgical interventions. We present a case of a 6-year-old patient with an embryonal tumor of the central nervous system. Following a craniotomy for the resection of the tumor, an external ventricular drainage was placed. Several weeks after surgery, she developed signs of meningism. Cerebrospinal fluid cultures were positive for pan-drug resistant Acinetobacter baumannii. Several revisions with the insertion of new external valves were done. She was treated with intravenously meropenem and vancomycin combined with colistin administrated intraventricularly. Significant improvement was seen clinically with negative cultures after 2 weeks. The synergistic action of colistin administrated locally combined with systemic antibiotics may be a promising option for critically ill patients with pan-drug resistant A. baumannii central nervous system infection. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, ANTIBIOTICS: CURE AND RISK FACTOR FOR CLOSTRIDIOIDESDIFFICILE INFECTION(Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 2024-04-22); ; ; ; Introduction: The major risk factor for acquiring an infection with Clostridioides difficile (CDI) is a long-term antibiotic treatment. Contrarily, the treatment of severe CDI cases involves application of antibiotics like vancomycin or metronidazole. Our aim was to investigate the percentage of resistance to eight antibiotics (vancomycin, metronidazole, tetracycline, clindamycin, erythromycin, imipenem, ciprofloxacin and moxifloxacin) among Clostridioides difficile isolates, indirectly evaluating the risks of acquiring CDI and the risks of therapeutic failure. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Attrition in Everyday Dental Clinical Practice(Macedonian Academy of Sciences and Arts/De Gruyter, 2024-12); ; Introduction Attrition, its prevention and therapy is a complex problem, with a multifactorial etiology. The aim of this paper is to examine the treatment of attrition in everyday dental clinical practice with the help of soft inserts. Material and method In this study, 30 patients were included, divided into two groups of 15 patients, the study and the control group. The examined patients had first degree of attrition diagnosed according to the Bardsley index, after which the condition was noted in four intervals: the initial situation, after 2, 4 and 6 months from the first visit. The research data were processed in Statistica for Windows 7.0 and SPSS version 20, and the same were presented graphically. Results In the study group with I degree of attrition, for p>0.05, no statistically significant difference was determined in the range of the Bardsley index between the four measurement times (Friedman Test: N=15; Chi-Square=7,200; df =3; p=0.0658). In the control group, for p<0.05, a significant difference was determined in the range of the Bardsley index between the four measured times (Friedman Test: N=15; Chi-Square=15.180; df=3; p=0.0017) with a significantly high value after 6 months. Discussion From the results obtained when comparing the study group in patients with I degree of attrition and the control group, a negative change from the parafunction bruxism was observed in 20% of the test group and 47% of the control group. The results of our research indicated that in patients with the first degree of attrition from the study group, soft dental inserts give significantly better results than untreated patients in the control group. This correlates with the research of Khayat N. et al. Conclusions From the results of the subjects in the group with I degree of attrition, we can conclude that the soft inserts has great efficiency as a means of management of this parafunction and therefore its application in daily therapy is recommended. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, VAGINAL MICROBIOTA COMPOSITION PROFILES IN WOMEN AT DIFFERENT LIFE STAGES(Macedonian Association of Anatomists and Morphologists, 2024); ; ; The vaginal microbiome is a reproductive organ-specific association that harbors a unique collection of anaerobic and aerobic microorganisms. It plays a crucial role in woman`s reproductive wellbeing and prevention of urogenital diseases including bacterial vaginosis (BV), infections with opportunistic microorganisms, yeast infections, urinary tract infections and sexually transmitted diseases. The composition of the vaginal microbiota undergoes significant changes during different stages of women's life cycle and is influenced by variations in sex hormone levels, physiological factors (e.g. the menstrual cycle and pregnancy) and individual lifestyle choices. Evidence shows that these various factors can influence the vaginal microbiome, potentially leading to an imbalance in the microbial community and genital infections. The aim of the present study was to compare the composition of vaginal microbiota in women at different life stages. A total of 2032 vaginal and cervical swab samples from women at different age, submitted for routine testing at the Institute for microbiology and parasitology were analyzed. Vaginal microbiota was evaluated by means of quantitative PCR in real time (Femoflor Screen, DNA-Technology).The results showed a dominance of lactobacilli in majority of the samples from the first two groups young women and women in reproductive years (72% and 69%, respectively), whilst a decrease of normocenosis was noted in women over 45 years (47%). Moderate dysbiosis was detected in 18% of both 15-25 yrs and 25-45 yrs age groups compared to 21% of the women of the > 45 yrs group. Severe dysbiosis prevailed among women of the >45 yrs group (32%) compared to 13% and 10% in the 15-25 yrs and 25-45 yrs age groups, accordingly. The results from this study provide comprehensive picture of our current knowledge of the composition and abundance of the microbiota of the female reproductive tract during different life phases. The tremendous importance of the microbiome for the reproductive health imply a necessity of future studies focused on providing more detailed information its composition and susceptibility to external influences. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, RAISING AWARENESS OF MYIASIS: A CASE SERIES FROM AUTOCHTHONOUS AND IMPORTED INFESTATIONS(Ss. Cyril and Methodius University in Skopje, Medical Faculty - Skopje, 2025-05-01); ; ; ; Myiasis is an infestation of the tissues of livinghumans and other vertebrates by the immature stages (larvae) of Diptera. According to the International Classification of Diseases, myiasis belongs to the morbidity classification ICD-10-CM, with the ICD code B87 (2025 edition). Myiasis is not mandatory to report, and through our experience, we aimed to raise awareness of this infestation among medical practitioners and the public. We discuss seven cases of myiasis diagnosed over five years (2019-2024) whose specimens (larvae) was submitted to the Institute for Microbiology and Parasitology, Medical Faculty - Ss. Cyril and Methodius University in Skopje, Republic of North Macedonia. The collected maggots were analyzed through macroscopic and microscopic examination. Morphological identification of the larvae was conducted using identification keys. Photographs were taken using an Olympus SZX9 and a Carl Zeiss Stemi 508 stereo microscope with an integrated high-resolution digital camera. Our findings confirmed an infestation by larvae from two autochthonous species, Sarcophaga argyrostoma and Lucilia sericata. We also identified an imported fly species, Cordylobia anthropophaga. The larvae can cause various symptoms depending on their relationship with the host and the anatomical location in the body. The outcome is related to comorbidities and the provision of appropriate, timely treatment. Medical practitioners should consider myiasis in their differential diagnosis. Nosocomial myiasis is a significant concern and requires special attention and preventive measures.