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    Item type:Publication,
    Few case studies as ideas for zero-waste from food production and processing
    (University of Sarajevo, Institute for Genetic Engineering and Biotechnology, 2023-12-20)
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    Svetozarevic Arsovic, Milica
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    Saiti Musliji, Zimere
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    Chadikovski, Aleksandar
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    Abazi Bajrami, Drita
    Globally, the amount of agricultural waste is huge but not properly utilized yet. Precisely, about one billion tons of food produced for human consumption is wasted each year. This wastage global economy costs are estimated at US$ 1 trillion on annual level. The definition of food waste varies globally depending on where food waste occurs in the food supply and consumption chain, how it is generated, and what it covers. This review is a compilation of few different approaches in the context of zero waste from food production and processing. Soybean hull exploitation due to mass production of tofu, soymilk, edamame, tofu sausages and burgers, soy breads, soy pasta and soymilk yoghurts and cheeses, animal feed leads to a formation of high waste load. There are several available ways for soybean hull valorization: as a biofertilizer, as a substrate for microbial growth, as an adsorbent, for extraction of antioxidants and in our case for extraction of enzymes. The global consumption of coffee is approximately 10 million tons, resulting in a substantial production of spent coffee grounds (SCG) worldwide. However, this by-product has recently gained attention as a valuable source of usable compounds, particularly in our case for essential oils. During the cheese production process, the whey obtained as a by-product can be used as a raw material for development of new dairy products and in the same time to prevent the potential pollution of natural watercourses. The valorisation of the waste whey obtained in the cheese production by using it in the development of new products also contributes in the environment protection. In N. Macedonia, about 1000 tons of blood waste from industrial slaughterhouses flow into natural watercourses every year and it can find application in various industries. These are just a few examples that show that the possibilities for the implementation of the zero-waste concept are unlimited.
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    Item type:Publication,
    DNA microsatellite analysis for tomato genetic differentiation
    (National Library of Serbia, 2015)
    Miskoska-Milevska, Elizabeta
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    Bandzo, Katerina
    Commonly used method for determination of the genetic diversity among the populations is the test for genetic differentiation. DNA microsatellite markers are usually used to investigate the genetic structure of natural populations. The aim of this study was to evaluate the applicability of eight DNA microsatellite loci (LECH13, LE21085, LEMDDNa, LEEF1Aa, LELEUZIP, LE20592, TMS9 and LE2A11) in genetic differentiation of six morphologically different tomato varieties (var. grandifolium from subsp. cultum; var. cerasiforme - red and yellow, var. pruniforme and var. pyriforme from subsp. subspontaneum; and var. racemigerum from subsp. spontaneum). The fragment analyses was performed using Applied Biosystems DNA analyzer (ABI 3130) and GeneMapper® Software program. The data were analysed using the specific program Power Marker Software. The average number of detected alleles was 3,625. Also, the average PIC value for all 8 DNA microsatellites loci was 0,3571. The genetic differentiation test in the researched tomato subspecies showed minor differentiation for locus LELEUZIP (- 0,0009), modest differentiation for locus LECH13 (0,0896), locus LEMDDNa (0,0896) and locus LE21085 (0,0551) and major differentiation for locus LE2A11 (0,7633), locus LEEF1Aa (0,6167), locus TMS9 (0.4967) and locus LE20592 (0,4263). On the other hand, in the estimated tomato varieties, locus LE21085 (0,0297), locus LECH13 (0,0256) and locus LELEUZIP (0,0005) showed minor differentiation, locus LEMDDNa (0,1333) showed modest differentiation, while locus TMS9 (0,5929), locus LEEF1Aa (0,5006), locus LE2A11 (0,4013) and locus LE20592 (0,2606) showed major differentiation. The eight DNA microsatellite loci can be applicable solution for tomato genetic differentiation. The overall results suggest that these microsatellite loci could be used in further population genetic studies of tomatoes.
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    Item type:Publication,
    ACTN3 association on maximal muscle power, after 6 weeks of power training
    (Faculty of physical education sport and health, 2021-06)
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    The research tried to discover/find out [1] whether the success of transformation of max muscle power will be just because of the training sessions? Or perhaps [2] the genetic potential of examinees will be important to make bigger and faster transformation of max muscular power. The experimental program was applied within (N) 21 examinees, age of 18-20 years, non-athlete population, for a period of 6 weeks. The programme included exercises for transformation of the maximal strength component of the flexors and extensors muscles on the elbow of the examinees' non-dominant arm (load- 90-95% of one repetition maximum). Three tests for one repetition maximum (flexion+ extension) were performed (program start, 3th and 6th week). Mutation of ACTN3 genotype allele, of the examinees, were analysed, according to establish three types of examinees: (type I) slow-oxidative - XX type/group, (type IIA) fast oxidative/glycolytic RX type/group and (type IIB) fast glycolytic -RR type/group. The three groups of examinees (XX, RR, XR) do not differ in terms of the achievements of one repetition maximum (flexors/extensors) after 3th and after 6th week. The genetic predisposition of the muscle tissue for strength capacities is not always decisive for the transformation of the maximal strength abilities.
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    Item type:Publication,
    Influence of the fertilizers Slavol and Biohumus on potato leaf area and stomatal density
    (University of Kragujevac - Faculty of Agronomy, Čačak, 2020)
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    Dimovska, Daniela
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    Iljovski, Igor
    Different types of fertilizers, such as chemical fertilizers, organic fertilizers and natural fertilizers are available on the market. The type of fertilizer has a large impact on the plant production quality. The aim of this study, which was conducted in field conditions in the village Jurumleri, near to Skopje, North Macedonia, was to investigate the influence of the application of two different types of fertilizers on leaf area and stomatal density in potato. The influence of microbiological fertilizer Slavol and organic fertilizer Biohumus was investigated. Also, a control plot without any fertilizer was used. A statistically tool (ANOVA test) was used for data analyses. From the results, it can be concluded that the statistically highly significant difference were found between average leaf area of plants treated with Slavol and plants treated with Biohumus (p<0.001). Also, statistically highly significant difference between plants treated with Slavol and control plants regarding total stomatal density was detected (p<0.001). Between plant treated with Slavol and plants treated with Biohumus was noticed statistically very significant difference (p<0.01). It can be concluded that the microbiological fertilizer Slavol showed better results than Biohumus in our research.