Pavlovska, Kristina
Preferred name
Pavlovska, Kristina
Official Name
Pavlovska, Kristina
Main Affiliation
Email
kristina.pavlovska@medf.ukim.edu.mk
23 results
Now showing 1 - 10 of 23
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Item type:Publication, Costs of treating serious adverse effects of drugs used for treatment of obesity: comparison of selected European countries(Informa UK Limited, 2024-11-03) - Some of the metrics are blocked by yourconsent settings
Item type:Publication, High Performance Liquid Chromatographic Method for Direct Determination of Diazepam in Whole Blood and Serum - Optimization of Solid-Phase Extraction Method(Macedonian Academy of Sciences and Arts / Walter de Gruyter GmbH, 2017-12-01); ; ; ; Herein, we present a simple and rapid high performance liquid chromatographic (HPLC) method with UV-detection for the direct determination of diazepam in whole blood and serum that can be used for monitoring diazepam levels in clinical samples analysis. The isolation of diazepam and the internal standard bromazepam from serum and whole blood samples was performed using solid phase extraction method with RP select B cartridges. The analytes were separated employing a reversed phase C8 column with a mobile phase composed of 0.1 % (V/V) triethylamine in water (pH 3.5) and acetonitrile (63:37, V/V). UV detection was carried out at 240 nm. Linearity was achieved in the range from 10.0-1000.0 ng/ml for serum and whole blood. The method was applied to spiked and real biological samples after an oral administration of 10 mg diazepam. In conclusion, the proposed method is simple, rapid and provides efficient clean-up of the complex biological matrix and high recovery of diazepam. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A New Solid-Phase Extraction Method for Determination of Pantoprazole in Human Plasma Using High-Performance Liquid Chromatography(ID Design 2012/Scientific Foundation SPIROSKI, 2019-06-15); ; ; ; A new simple, selective and accurate high-performance liquid chromatographic (HPLC) method utilising solid-phase extraction for the determination of pantoprazole in human plasma samples has been developed. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Cranberry, a potential alternative treatment for urinary tract infections(Macedonian Association of Anatomists, 2023); ; ; ; Radomir JovchevskiVaccinium macrocarpon is perennial plant traditionally used as an herbal medicine in treatment and prevention of UTIs. Although the mechanisms of action are not jet fully understood it is presumed that they involve interferation with bacterial adhesion and changes in bacterial morphology mainly attributed to the plants proanthocyanidins. C ranberry extracts (CE) standardized for different concentrations of proanthocyanidinnes (PACs), CE in combination with antibiotics (norfloxacin and vancomy cin) and antibiotics alone (only antibiotics) were investigated for their effect on different strains of uropathogenic E.coli, S. saprophyticus and E. faecalis . As a source of CE we used commercial herbal supplements containing only Vaccinium macrocarpon extract (37.5 mg PACs) or CE in combination with D - manoza (25, 3 mg PACs). We used bacterial strains isolated from out patients with UTI s reffered for routine urine examination at the Institute of microbiology and parasitology. Sensitivity of the pathogen s to CE (as monoagent or combined in herbal mix) was evaluated with disc diffusion method. Our results showed stronger effect of CE on the growth of E.coli compared to G ram - positive strain s . S. saprophyticus strains were more susceptible to the extract/herbal mixes compared to the enterococci which predominantly presented as recalcitrant to the inhibitory activity of cranberry/herbal mixes. The sample size of this study was small to draw definite conclusions but our results illuminate avenues for future re search of the potential of cranberry as an alternative treatment in patients with UTIs. - Some of the metrics are blocked by yourconsent settings
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Item type:Publication, Comparative, Single-Dose, 2-Way Cross-Over Bioavailability Study of Two Olanzapine 10 Mg Tablet Formulations in Healthy Volunteers Under Fasting Conditions(Macedonian Academy of Sciences and Arts/Walter de Gruyter GmbH, 2022-07-13); ; ; ; Objectives: Olanzapine is an atypical antipsychotic that is approved across Europe, the USA, and in many other countries for oral treatment of schizophrenia and acute manic episodes in patients with bipolar disorder as well as for maintenance therapy to prevent recurrence in responders. The objective of the present study was to compare the pharmacokinetics of two 10 mg tablet formulations of Olanzapine following a single oral dose in healthy volunteers under fasting conditions, as per the European Medicine Agency (EMA) guidelines to grant marketing authorization. Methods: This study was a randomized, open-label, two-treatment, two-period, two-sequences, single-dose, cross-over design with a washout period of 14 days. Both the test and the reference products were administered as 10 mg tablets with 240 mL of water after an overnight fast in each study period. A total of twenty blood samples were collected before dosing and within 144 hours after drug administration. Adverse events were monitored, recorded, and evaluated by investigators throughout the study. Results: Of the 24 healthy adult male subjects enrolled, all of them completed both study periods. The geometric mean ratio 90% confidence intervals (CI) for fasting Cmax, AUC0-t, and AUC0-infinity were 94.83-113.71%, 95.04-105.69% and 95.94-107.00%, respectively. The 90% CI for the ratios of the three primary pharmacokinetic parameters (using log-transformed data) were within the range of 80-125%, meeting the regulatory criteria for bioequivalence. Conclusions: The generic Olanzapine was bioequivalent to the reference formulation. It was well tolerated and provides an acceptable alternative to the reference drug. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, COVID-19 disease induced alteration of oxidative stress and clinical laboratory parameters in moderate and severe patients(2022-08); ; ; ; The aim of our study was to present the clinical alterations of CRP, LDH, neutrophil to lymphocyte ratio, platelets to lymphocyte ratio, D-dimer, blood gas analyses, vitamin D, VEGF, IL-6, IFN-γ, CD4+, CD8+) and their correlation with oxidative stress index (OSI) in hospitalized COVID-19 patients. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Importance of 6-Thioguanine Nucleotide Metabolite Monitoring in Inflammatory Bowel Disease Patients Treated with Azathioprine(Macedonian Academy of Sciences and Arts / Walter de Gruyter GmbH, 2019-05-01); ; ; ; Ribarska, Jasmina TonicThe active metabolite of azathioprine, 6-thioguanine nucleotide (6-TGN) is the main component responsible for the immunosuppressive effect in treatment of inflammatory bowel disease (IBD). The aim of this study was to assess the correlation between the concentration of 6-thioguanine nucleotide and disease activity, azathioprine-related adverse effects and time duration of treatment in patients with inflammatory bowel disease. Thirty-four patients were included in this study. Type of disease, gender, time duration of therapy and adverse effects were recorded. Metabolite concentration was determined by high performance liquid chromatography. Twenty-one percent of patients have experienced an adverse effect, with leucocytopenia most commonly occurring (42.9%). More adverse effects were registered when patients were treated with azathioprine in a period of less than 3 months in comparison to the group of patients that have been under therapy between 3-12 months and more than 12 months (p˂0.05). Most of the patients that presented any adverse effect had high 6-TGN concentration (>450 pmol/8x108 Er). The mean value of 6-TGN metabolite concentration in IBD patients treated with azathioprine was 437.46 pmol/8x108 Er ± 198.82 pmol/8x108. The time duration of azathioprine treatment did not have any significant impact on the achieved 6-TGN concentration (p>0.05).Twenty patients (58.9%) had achieved remission after therapy initiation with azathioprine. More alertness is recommended to clinicians towards patients in the first 3 months of the therapy. Our study demonstrated that higher 6-TGN concentration is associated with azathioprine toxicity. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Protective Effects of At1-Receptor Blocker and Ca Antagonist Combination on Renal Function in Salt Loaded Spontaneously Hypertensive Rats/ Протективни Ефекти На Комбинацијата На Ат1 Рецепторен Блокатор И Калциум Антагонист Врз Реналната Функција Кај Спонтано Хипертензивни Стаорци Оптоварени Со Сол(Macedonian Academy of Sciences and Arts/Walter de Gruyter GmbH, 2015-05-01); ; ; ; Salt sensitive hypertension is known to be a contributing factor for the progression of kidney disease. This study was undertaken to investigate the role of excessive dietary salt on renal function and to evaluate the effect of valsartan and amlodipin given as a combination therapy on blood pressure and parameters specific to the renal function in salt loaded SHR rats. 48 male SHR rats at age of 20 weeks and body weight ranging between 270-350 g were used. SHR rats were divided into 3 groups: control group of rats -SHRC (n = 16) given tab water ad libitum and two salt treated groups in which tab water was replaced with a solution of NaCl (1%) from age of 8 weeks given ad libitum: SHRVAL+AMLO group (n = 16) where investigated drugs were administered at a dose of 10 mg/kg/ b.w. (valsartan) and 5 mg/kg/ b.w. (amlodipin) by gavage and SHR NaCl group (n = 16) that received saline in the same volume and the same time intervals as the SHRVAL+AMLO group. For a period of 12 weeks we have investigated the effect of the VAL+AMLO drug combination on systolic blood pressure (SBP), body weight and renal function tests. Salt loading with 1% solution in the SHR NaCl group has lead to significant increase of blood pressure, proteinuria and decrease in creatinine clearance. Combined treatment with АТ1-receptor blocker and calcium antagonist has managed to control blood pressure and ameliorated renal damage.</jats:p> - Some of the metrics are blocked by yourconsent settings
Item type:Publication, DO LACTOBACILLI CHALLENGE GARDENELLA VAGINALIS BIOFILMS?(Macedonian Association of Anatomists, 2020); Bacterial vaginosis (BV) is a highly prevalent vaginal dysbiosis that has been linked to adverse pregnancy outcomes and enhanced transmission of sexually transmitted infections (STIs). Key characteristics of the disease process are thought to be depletion of vaginal Lactobacillus and overgrowth of anaerobes (often dominated by G.vaginalis) and a pH > 4.5. Currently, is consensual that BV also involves the presence of a dense, structured and polymicrobial biofilm, primarily constituted by G.vaginalis clusters, strongly adhered to the vaginal mucosal surface. Biofilms are communities of microorganisms attached to a surface and encased in a selfproduced polymeric matrix. Reduction of the adhesive and biofilm forming capacity activity of G. vaginalis bacteria by Lactobacillus strains is a well-known and desired effect of strains for potential vaginal probiotic application. The objectives of the present study were to evaluate in vitro the effect of Lactobacillus on biofilm production by different species of G.vaginalis isolated from women with bacterial vaginosis (BV). A total of 36 isolates from women with BV identified as G.vaginalis were tested for their biofilm-forming capacity as monocultures and in bacterial coculture with confirmed non-biofilm producing strain of Lactobacillus, in a ratio of 1:1 by microtiter plate assay. Lactobacillus strain in our study was capable of interfering with the growth of G. vaginalis biofilms to different degrees. According to the criteria for biofilm-forming ability, after 24-h incubation 25%, 28% and 22% of Gardnerella monocultures were strong, moderate and weak biofilm producers, compared to 5.5%, 14% and 33.5% of Gardnerella+Lactobacillus cocultures, respectively. Our results indicate the potential of lactobacilli as probiotics, since they effectively reduced the adheration and biofilm formation of the tested Gardnerella species which is a well-known and desired effect of strains for potential vaginal probiotic application. Key words: biofilms, resistance, lactobacilli, genital. Bacterial vaginosis (BV) is a highly prevalent vaginal dysbiosis that has been linked to adverse pregnancy outcomes and enhanced transmission of sexually transmitted infections (STIs). Key characteristics of the disease process are thought to be depletion of vaginal Lactobacillus and overgrowth of anaerobes (often dominated by G.vaginalis) and a pH > 4.5. Currently, is consensual that BV also involves the presence of a dense, structured and polymicrobial biofilm, primarily constituted by G.vaginalis clusters, strongly adhered to the vaginal mucosal surface. Biofilms are communities of microorganisms attached to a surface and encased in a selfproduced polymeric matrix. Reduction of the adhesive and biofilm forming capacity activity of G. vaginalis bacteria by Lactobacillus strains is a well-known and desired effect of strains for potential vaginal probiotic application. The objectives of the present study were to evaluate in vitro the effect of Lactobacillus on biofilm production by different species of G.vaginalis isolated from women with bacterial vaginosis (BV). A total of 36 isolates from women with BV identified as G.vaginalis were tested for their biofilm-forming capacity as monocultures and in bacterial coculture with confirmed non-biofilm producing strain of Lactobacillus, in a ratio of 1:1 by microtiter plate assay. Lactobacillus strain in our study was capable of interfering with the growth of G. vaginalis biofilms to different degrees. According to the criteria for biofilm-forming ability, after 24-h incubation 25%, 28% and 22% of Gardnerella monocultures were strong, moderate and weak biofilm producers, compared to 5.5%, 14% and 33.5% of Gardnerella+Lactobacillus cocultures, respectively. Our results indicate the potential of lactobacilli as probiotics, since they effectively reduced the adheration and biofilm formation of the tested Gardnerella species which is a well-known and desired effect of strains for potential vaginal probiotic application.
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