Bitoljanu, Natasha
Preferred name
Bitoljanu, Natasha
Official Name
Bitoljanu, Natasha
Main Affiliation
Email
natasha.bitoljanu@medf.ukim.edu.mk
27 results
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Item type:Publication, INFORMED CONSENT IN GENETIC REASEARCH(Macedonian Association of Anatomists and Morphologists, 2023); ; ; ; Recognizing the ethical, legal, and social implications (ELSI) of genetic testing becomes crucial for physicians in the face of complex medical issues, as they are increasingly expected to counsel their patients regarding the medical, psychological, and social responses arising from genetic information. Genetic medicine, with its extreme complexity and the potential repercussions on an individual's life, raises important questions in the ethical, deontological, and legal realms of medicine, playing a primary role in personalized medicine. The aim of this paper is to underscore the significance of informed consent and to provide insights into the ethical procedures associated with genetic testing. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Consent form, the highest ethical standard in creating DNA databases for criminal investigation(Elsevier BV, 2022-12); ; ; Janevski, RobertPreparation of DNA databases for the purpose of criminal investigation opens discussions about ethical-legal issues concerning violation of human rights. The practice shows that one of the human rights that can be misused while creating such a database is the right to freedom, the freedom to make a decision. When preparing DNA databases, the right to freedom refers to free decision of the person to be sampled for carrying out the test. The right to freedom and respect to self-determination of the person implies the necessity of prior consent of the subject when preparing a database on general population level. This is not a case when databases are created from persons under investigation for committing a crime, who are compulsory subjected for obtaining samples for DNA analysis. Legal regulations approve the duty of the police and its authorization in collecting samples for personal or criminalistic identification, analyzing, keeping and eliminating collected personal information when criminal prosecution is concerned. In these cases, consent form from the subject is not necessary. However, we should be aware that the process of taking and collecting of personal information by the national institutions can have direct impact of privacy of the subject, no matter if this information is going to be used or not. In purpose of fair balance between public and private interest, consent form can be redefined and the person from whom the biological material is provided will be unequivocally made aware of the purposes for which his genetic data will be used, how long his DNA will undergo further automated processing, and about the procedure and under what conditions his DNA profile can be removed from the national DNA databases. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, TIME-DEPENDENT EXPRESSION OF IL-1β mRNA - A PRELIMINARY STUDY FOR POSSIBLE WOUND AGE MARKER(Macedonian Association of Anatomists and Morphologists, 2023); ; ; ; Siamkouri, RosicaAccurate estimation of wound age is a very important task in forensic medicine field, because it can helps in reconstruction of crime scene. IL-1β is proinflamatory cytokine and plays a major role in wound healing process. The aim of the study is to develop a method for the reliable estimation of dermal injury age. In this study the expression of IL-1β mRNA was evaluated by real-time PCR at skin human wounds with different post-injuried period. We collected 27 human skin wound samples from forensic autopsy cases at our Institute. In our study were include samples from lacerations, incised wounds and stab wounds, with a known time of injury and death. Wound samples were divided into five groups: control group (n=5); first group consisted of cases with immediate death (n=7); second group consisted of cases with survival from 1 hour to 6 hours (n=5); the third group comprised cases with survival from 6 hours to 72 hours (n=3); the fourth group comprised cases with survival from 72 hours to 168 hours (n=7). In this study we detected increased expression levels of IL-1 β mRNA in the second group with survival time from 1 to 6 hours. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Age identification in children and youngsters(Slovenska Sudnolekarska Spolovnost Slovenskej Lekarskej Spolocnosti, 2017-05); ; ; ; - Some of the metrics are blocked by yourconsent settings
Item type:Publication, A new method to determine wound age in human skin injuries(Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, 2023-06-16); ; ; ; Chakar, LjupcoWound age determination is a significant part of everyday practice in forensic medicine. Determining the wound age answers a large number of questions arising from a criminal-legal event. The RT-PCR technique enables qualitative and quantitative analysis of mRNA and can register minimal changes in gene expression, which makes it more sensitive and accurate compared to the immunohistochemical method. The study included 51 wound samples of autopsy cases. In this study we conducted comparative analyses for different methods of homogenization and isolation. An analysis was carried out for the selection of a suitable reference gene that will be used for normalization of the expression levels of the investigated target gene. Two reference genes, GAPDH and 18S, were analyzed in the study. A biphasic expression of IL-6 information RNA (mRNA IL-6) was determined in relation to the other groups, including the control group. 18S has better efficiency of amplification then GAPDH. RNA was isolated from all examined tissues with the TriXact kit on the MagCore Plus II instrument. It can also be concluded that mechanical maceration of the tissue was more effective than the other methods of homogenization. 18S is a more stable reference gene for the analysis of skin samples. IL-6 can could be used as a marker in wound age determination. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Morphology of microglia in cerebral contusions(Macedonian Association of Anatomists and Morphologists, 2015); ; ; ; - Some of the metrics are blocked by yourconsent settings
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Item type:Publication, Forensic aspect of cerebral contusions(Macedonian medical association/De Gruyter, 2014-08); ; ; Introduction. Contusions are injuries of the brain dominantly incurred by blunt-force trauma in the head (blow, impact, fall). Proper analysis of the cerebral contusions within all head injuries and data case gives us complete view of the event and enables us to choose the right direction in the investigation. The aim of this investigation was to perform a forensic medical analysis of cases with brain injury (cerebral contusions) incurred by blunt-force trauma, and to compare results with a prior extensive research done at the Institute of Forensic Medicine, Skopje. Methods. A total of 76 cases with cerebral contusions incurred by blunt-force trauma were included. The following data were analyzed: gender, age, event, skull fractures, type of fracture, localisation of contusions and toxicological analysis. The basic descriptive processing included the above information-variables taken from the written reports of the performed autopsies. The data are displayed graphically with the use of Microsoft Excel 2007. Results. Male was the dominant gender in the analyzed cases (86%), with mean age of 51 years. Traffic accidents were the main factor for these injuries (76%). Skull fractures were found in 67% of cases. The dominant cerebral lobes were the temporal and the frontal lobe, and the contrecoup contusions were found in 55% of cases. Conclusions. The experience gained during the processing and analysis of these brain injuries answers the most complicated questions, and contributes to the healthcare especially in dealing with the diagnosis and treatment of cerebral contusions. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Importance of quality control in sample preparation workflow of forensic degraded samples for massively parallel sequencing (MPS)(IAR Publishing, 2023); ; ; ; Molecular genetic analyses occupy a significant part of laboratory tests in forensic practice. Pre-prepared commercial kits used for DNA extraction are an excellent choice for samples provided by living people, but in forensic practice, most of the samples that undergo molecular processing are often degraded and may pose a challenge in the laboratory. A special challenge is to provide quality genetic material from post-mortem samples that would be suitable for further analysis with massively parallel sequencing. The aim of this paper is to evaluate the applicability of two silica-based DNA extraction methods for post-mortem blood samples with varying storage time and degrees of degradation, and also, to establish proper quality control in sample preparation workflow for Massively Parallel Sequencing using Ion Torrent GeneStudio™ S5 platform. The study was performed on 48 blood samples with different storage time. Silica-based DNA extraction protocols was applied and quantification was made using Qubit 3.0 fluorimeter, followed by Real-Time PCR quantification. The results indicate high variability between the obtained DNA quantities from post-mortem blood samples with different storage time. Correlation was determined between storage time and sample quantity and quality. The Qiagen Mini Kit & Micro Kit are applicable for DNA extraction from blood samples with various storage periods and degradation levels, which can be used for further NGS analyses after careful quality control and optimization of library preparation conditions. - Some of the metrics are blocked by yourconsent settings
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