Institute of Animal Science

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    Genetic structure and distribution of oxynoemacheilus bureschi (balitoridae, teleostei), and its phylogenetic relationships with other European stone loaches
    (2008)
    Šedivá, Alena, Apostolou Apostolos, Janko Karel, Kohout, Jan, Kostov Vasil, Sanda Radek
    The genetic differentiation of Oxynoemacheilus bureschi (Drensky, 1928) from all three drainages (Struma=Strymon, Mesta=Nestos, Vardar=Axios) where this species occurs, as well as its phylogenetic relationships with other European stone loaches, was assessed using the mitochondrial cytochrome b gene. The phylogenetic reconstruction revealed two distinct clades within the European stone loaches with high bootstrap values. Very low genetic variability with no internal haplotypic structure has been found between and within all examined O. bureschi populations, indicated by low polymorphism and similar haplotypes. According to the nesting design and demographic patterns, the range of O. bureschi was not constant, but underwent expansion in the recent past. Lack of variation, a rather unusual phenomenon for fishes from the southern Balkans, is ascribed to facilitation of dispersal due to seashore regression, confluence events and river capture during the Pleistocene.
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    Effects of isoflavones in feed on plasma estradiol concentration and productive performances in laying hens
    (2016)
    Gjorgovska Natasha, Filev Kiril, Levkov Vesna, Kostov Vasil, Nastova Rodne
    Isoflavones are phytoestrogens which have estrogen-like activity in animals and humans with structure similar to 17-β-estradiol and capable of binding to estrogen receptors (ERs).This experiment was conducted to demonstrate the effect of isoflavones on estradiol concentration in blood and its influence on the productive performances in ISA Brown laying hens fed with supplemented feed with large amount of isoflavones. Isoflavones as non steroid phytoestrogens were added in large amounts to the feed of laying hens in 0, 1000, 2000 and 3000 mg kg-1 of feed. Estradiol concentration in blood of the isoflavone-treated chickens was significantly higher in comparison with the control group (P < 0.01). The supplemented isoflavones in the feed have significant improvement on the egg weight during the 1st month (P < 0.05) and 2nd month (P < 0.01) and also for the whole experimental period (P < 0.05). Further studies are required to investigate the effect of isoflavones on other reproductive hormones (follicle stimulating hormone (FSH) and progesterone) which are related with the egg production. © 2010 by Islamic Azad Univerty, Rasht Branch, Rasht, Iran.
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    The effect of feed rich in DHA on egg production, egg components and DHA content in yolk
    (Bulgarian Journal of Agricultural Science, 2016)
    Gjorgovska Natasha Filev K.,Levkov V. Gjorgjievski S., Kostov Vasil
    The effect of diets rich with DHA (docosahexaenoic acid) on egg production, the influence on egg structural components and DHA deposition in egg yolk were investigated on Hisex Brown laying hens. The experimental groups were fed with diets rich with 792 mg DHA kg-1 and 1180 mg DHA kg-1. The control group received commercial diets with no content of DHA. Throughout the experiment there were no statistically significant differences in live weight of laying hens (g) and feed conversion ratio (FCR:g) between control and experimental groups (P>0.05). The structural components of eggs (egg weight, white weight, yolk weight and shell weight) were also not statistically significant (P>0.05), but only the egg yolk:white ratio was statistically different (P<0.01). The concentration of DHA in 100 g yolk was 818 mg and 974 mg in group 2 and 3, respectively. The transfer of DHA was significantly higher in the experimental groups fed with supplemented diet with higher amount of DHA (P<0.01). © 2016, National Centre for Agrarian Sciences. All rights reserved.
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    Characterization and identification of selected metal-binding biomolecules from hepatic and gill cytosols of Vardar chub (Squalius vardarensis Karaman, 1928) using various techniques of liquid chromatography and mass spectrometry
    (Oxford University Press (OUP), 2019-06-19)
    Krasnići, Nesrete
    ;
    Dragun, Zrinka
    ;
    Kazazić, SnjeŽana
    ;
    Muharemović, Hasan
    ;
    Erk, Marijana
    Metals play crucial physiological roles, but they can also cause irreparable toxic effects through binding to important cellular biomolecules in aquatic organisms. The aim of this study was to determine the exact molecular masses and to identify several selected metal-binding biomolecules in hepatic and gill cytosols of Vardar chub (Squalius vardarensis Karaman, 1928). Methods applied for the achievement of this goal were SEC-AEC-HPLC for two-dimensional separation of cytosolic biomolecules, HR ICP-MS for metal measurements, and mass spectrometry (MALDI-TOF-MS and LC-MS/MS) for biomolecule mass determination and identification. The analyzed biomolecules included: Fe-binding biomolecules, which were identified as hemoglobin subunit β in the liver (molecular masses of ∼15 kDa), and hemoglobin subunits α and β in the gills (molecular masses of ∼11 kDa, ∼13 kDa and ∼15 kDa); heat-stable Cd-binding biomolecules, which were identified as MT isoforms MT-I and MT-II (molecular mass of ∼6.0 kDa in both liver and gills, and an additional 4.9 kDa isoform in the gills); and heat-stable Mo-binding biomolecules of molecular masses equal to 3.3 kDa (in the gills) and 8.5 kDa (in the liver). An important finding of this study was the obvious presence of different isoforms of the same biomolecules in the liver and gills. This was, among others, manifested through the absence of Zn binding to MTs only in the gills, indicating that the same type of biomolecule can be responsible for different functions in different organs. Thus, for better understanding of metal behaviour in aquatic organisms, it is crucial to identify cellular metal-binding biomolecules and their functions.
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    Influence of Genotypes, Age, Nutrition and Interactions Genotype X Nutrition on Mortality of Broiler Chickens
    (Institute of Animal Husbandry, Belgrade - Serbia, 2017)
    Nikolova, Nedeljka., Kocevski, Dragoslav., Nastova, Rodne., Puvaca, Nikola., Hrncar, Cyril., Kostadinov, Tosho
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    Major and Trace Elements in Raw Ewes’ Milk Used for Production of Traditional White Brined Cheese
    (National Agricultural and Food Center, Research Institute of Animal Production, 2017)
    Levkov, V., Stafilov, T., Pacinovski, N., Bacheva, K., Mateva, N., Gjorgovska, N., Eftimova, E., Kostadinov, T.