Please use this identifier to cite or link to this item:
http://hdl.handle.net/20.500.12188/23602
DC Field | Value | Language |
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dc.contributor.author | Makazlieva, Tanja | en_US |
dc.contributor.author | Vaskova, Olivija | en_US |
dc.contributor.author | Tripunoski, Toni | en_US |
dc.contributor.author | Miladinova, Daniela | en_US |
dc.contributor.author | Stojanoski Sinisa | en_US |
dc.contributor.author | Manevska, Nevena | en_US |
dc.contributor.author | Jakovski Z | en_US |
dc.contributor.author | Risteski, Sasho | en_US |
dc.contributor.author | Jovanovic H | en_US |
dc.contributor.author | Eftimov A | en_US |
dc.date.accessioned | 2022-10-19T07:47:36Z | - |
dc.date.available | 2022-10-19T07:47:36Z | - |
dc.date.issued | 2018-10 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12188/23602 | - |
dc.description.abstract | Introduction: Thyroid carcinoma (TC) comprises a spectrum of different tumors with wide range of biological behavior and prognosis. The techniques based on the latest trends in molec- ular biology may have application in diagnosis of metastatic TC. The aim of this study was to apply and analyze mRNA expres- sion of thyrotropin receptor gene (TSHR-mRNA) and thyroglob- ulin gene (Tg-mRNA) in peripheral blood of healthy individuals and patients with differentiated TC. Then to correlate between the total received 131 I therapy dose and expression levels of two evaluated genes. Material and Methods: In the study were included 57 subjects, consisting from 40 patients with TC and 17 healthy volunteers as a control group. Total RNA was isolat- ed from peripheral blood and used for two-step reverse tran- scriptase-PCR. Real time PCR was performed with appropriate primers. Relative quantification using 2-ΔΔCt method was applied. Results: TC patients with metastatic disease or loco-regional re- lapse expressed TSHR by a 8.57-fold higher level than healthy control individuals. TC patients with biochemical relapse ex- pressed TSHR by a 14.17-fold higher level than healthy controls, while expression of Tg was 6.6 -fold higher in TC patients with metastatic disease and loco-regional relapse than healthy con- trols and 8.34 -fold higher level compared with TC patients with excellent response to treatment. Conclusion: Our preliminary study showed that TSHR gene expression might have more useful application as a biomarker compared to detection of Tg gene expression. Positive non-significant correlation between ΔCt values for both target genes and total received dose of 131 I therapy with Spearman Rank was found. Key words: Thyroid carcinoma, TSH receptor gene expression, thyroglobulin gene expression | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer Science and Business Media LLC | en_US |
dc.relation.ispartof | European journal of nuclear medicine and molecular imaging | en_US |
dc.subject | thyroid carcinoma | en_US |
dc.subject | thyroglobulin gene expression | en_US |
dc.subject | tsh receptor gene expression | en_US |
dc.title | Determining thyrotropin receptor and thyroglobulin gene transcripts in peripheral blood of thyroid carcinoma patients and healthy individuals | en_US |
dc.type | Article | en_US |
dc.relation.conference | Annual Congress of the European Association of Nuclear Medicine October 13 – 17, 2018 Düsseldorf, Germany | en_US |
dc.identifier.doi | 10.1007/s00259-018-4148-3 | - |
dc.identifier.url | http://link.springer.com/article/10.1007/s00259-018-4148-3/fulltext.html | - |
dc.identifier.url | http://link.springer.com/content/pdf/10.1007/s00259-018-4148-3.pdf | - |
dc.identifier.url | http://link.springer.com/content/pdf/10.1007/s00259-018-4148-3.pdf | - |
dc.identifier.volume | 45 | - |
dc.identifier.issue | Suppl 1 | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
Appears in Collections: | Faculty of Medicine: Conference papers |
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