Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/8154
Title: Establishment of an EC50 database of pesticides using a Vibrio fischeri bioluminescence method
Authors: Efremova Aaron, Snezana
Katerina Tosheska Trajkovska 
Cekovska, Svetlana 
Aaron, Jean-Jacques
Issue Date: Aug-2019
Publisher: Wiley
Journal: Luminescence : The Journal of Biological and Chemical Luminescence
Abstract: An EC50 database was established to assess the acute toxicity of 16 PESTANAL pesticide standards and of seven pesticide commercial formulations using a Vibrio fischeri bioluminescence method. Half maximal effective concentration (EC50 ) is defined as the concentration of pollutant (in this case, pesticide) destroying 50% of the bacteria population and causing 50% bioluminescence inhibition, after a specified exposure time. Linear curves of bioluminescence inhibition versus pesticide concentration and EC50 values were obtained for exposure times (t) of 5 or 15 min for these pesticides. The EC50 values ranged from 6.90 × 10-4 to 0.83 mg/ml (t = 5 min), and from 9.00 × 10-4 to 0.37 mg/ml (t = 15 min) for pesticide standards, plus from 0.0077 to 0.74 mg/ml (t = 5 min), and from 0.0076 and 0.57 mg/ml (t = 15 min) for pesticide commercial formulations. The EC50 database allowed classification of the pesticides under study into three categories according to their toxicity: very toxic, toxic and moderately toxic. These results demonstrated that the establishment of an EC50 database and of linear curves of bioluminescence inhibition versus the pesticide concentration resulted in very important and irreplaceable tools to estimate the global and individual toxicity of pesticides present in environmental samples.
URI: http://hdl.handle.net/20.500.12188/8154
DOI: 10.1002/bio.3628
Appears in Collections:Faculty of Medicine: Journal Articles

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