Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12188/33394
Title: Comparative Proteomics Analysis of Urine Reveals Down-Regulation of Acute Phase Response Signaling and LXR/RXR Activation Pathways in Prostate Cancer
Authors: Davalieva, Katarina
Kiprijanovska, Sanja
Maleva Kostovska, Ivana
Stavridis, Sotir 
Stankov, Oliver 
Komina, Selim 
Petrushevska, Gordana 
Polenakovikj, Momir
Keywords: proteomics
non-invasive biomarker
prostate cancer
urine
LC-MS/MS
2-D DIGE/MS
Issue Date: 29-Dec-2017
Publisher: MDPI AG
Journal: Proteomes
Abstract: Detecting prostate cancer (PCa) using non-invasive diagnostic markers still remains a challenge. The aim of this study was the identification of urine proteins that are sufficiently sensitive and specific to detect PCa in the early stages. Comparative proteomics profiling of urine from patients with PCa, benign prostate hyperplasia, bladder cancer, and renal cancer, coupled with bioinformatics analysis, were performed. Statistically significant difference in abundance showed 20 and 85 proteins in the 2-D DIGE/MS and label-free LC-MS/MS experiments, respectively. In silico analysis indicated activation, binding, and cell movement of subset of immune cells as the top affected cellular functions in PCa, together with the down-regulation of Acute Phase Response Signaling and Liver X Receptor/ Retinoid X Receptor (LXR/RXR) activation pathways. The most promising biomarkers were 35, altered in PCa when compared to more than one group. Half of these have confirmed localization in normal or PCa tissues. Twenty proteins (CD14, AHSG, ENO1, ANXA1, CLU, COL6A1, C3, FGA, FGG, HPX, PTGDS, S100A9, LMAN2, ITIH4, ACTA2, GRN, HBB, PEBP1, CTSB, SPP1) are oncogenes, tumor suppressors, and multifunctional proteins with highly confirmed involvement in PCa, while 9 (AZU1, IGHG1, RNASE2, PZP, REG1A, AMY1A, AMY2A, ACTG2, COL18A1) have been associated with different cancers, but not with PCa so far, and may represent novel findings. LC-MS/MS data are available via ProteomeXchange with identifier PXD008407.
URI: http://hdl.handle.net/20.500.12188/33394
ISSN: 2227-7382
DOI: 10.3390/proteomes6010001
Appears in Collections:Faculty of Medicine: Journal Articles

Files in This Item:
File SizeFormat 
proteomes-06-00001.pdf1.96 MBAdobe PDFView/Open
Show full item record

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.