Determining thyrotropin receptor and thyroglobulin gene transcripts in peripheral blood of thyroid carcinoma patients and healthy individuals
Journal
European journal of nuclear medicine and molecular imaging
Date Issued
2018-10
Author(s)
Jakovski Z
Jovanovic H
Eftimov A
DOI
10.1007/s00259-018-4148-3
Abstract
Introduction: Thyroid carcinoma (TC) comprises a spectrum
of different tumors with wide range of biological behavior and
prognosis. The techniques based on the latest trends in molec-
ular biology may have application in diagnosis of metastatic TC.
The aim of this study was to apply and analyze mRNA expres-
sion of thyrotropin receptor gene (TSHR-mRNA) and thyroglob-
ulin gene (Tg-mRNA) in peripheral blood of healthy individuals
and patients with differentiated TC. Then to correlate between
the total received 131
I therapy dose and expression levels of two
evaluated genes. Material and Methods: In the study were
included 57 subjects, consisting from 40 patients with TC and
17 healthy volunteers as a control group. Total RNA was isolat-
ed from peripheral blood and used for two-step reverse tran-
scriptase-PCR. Real time PCR was performed with appropriate
primers. Relative quantification using 2-ΔΔCt method was applied.
Results: TC patients with metastatic disease or loco-regional re-
lapse expressed TSHR by a 8.57-fold higher level than healthy
control individuals. TC patients with biochemical relapse ex-
pressed TSHR by a 14.17-fold higher level than healthy controls,
while expression of Tg was 6.6 -fold higher in TC patients with
metastatic disease and loco-regional relapse than healthy con-
trols and 8.34 -fold higher level compared with TC patients with
excellent response to treatment. Conclusion: Our preliminary
study showed that TSHR gene expression might have more
useful application as a biomarker compared to detection of Tg
gene expression. Positive non-significant correlation between
ΔCt values for both target genes and total received dose of 131
I
therapy with Spearman Rank was found. Key words: Thyroid
carcinoma, TSH receptor gene expression, thyroglobulin gene
expression
of different tumors with wide range of biological behavior and
prognosis. The techniques based on the latest trends in molec-
ular biology may have application in diagnosis of metastatic TC.
The aim of this study was to apply and analyze mRNA expres-
sion of thyrotropin receptor gene (TSHR-mRNA) and thyroglob-
ulin gene (Tg-mRNA) in peripheral blood of healthy individuals
and patients with differentiated TC. Then to correlate between
the total received 131
I therapy dose and expression levels of two
evaluated genes. Material and Methods: In the study were
included 57 subjects, consisting from 40 patients with TC and
17 healthy volunteers as a control group. Total RNA was isolat-
ed from peripheral blood and used for two-step reverse tran-
scriptase-PCR. Real time PCR was performed with appropriate
primers. Relative quantification using 2-ΔΔCt method was applied.
Results: TC patients with metastatic disease or loco-regional re-
lapse expressed TSHR by a 8.57-fold higher level than healthy
control individuals. TC patients with biochemical relapse ex-
pressed TSHR by a 14.17-fold higher level than healthy controls,
while expression of Tg was 6.6 -fold higher in TC patients with
metastatic disease and loco-regional relapse than healthy con-
trols and 8.34 -fold higher level compared with TC patients with
excellent response to treatment. Conclusion: Our preliminary
study showed that TSHR gene expression might have more
useful application as a biomarker compared to detection of Tg
gene expression. Positive non-significant correlation between
ΔCt values for both target genes and total received dose of 131
I
therapy with Spearman Rank was found. Key words: Thyroid
carcinoma, TSH receptor gene expression, thyroglobulin gene
expression