Please use this identifier to cite or link to this item:
http://hdl.handle.net/20.500.12188/17528
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sophia David | en_US |
dc.contributor.author | Victoria Cohen | en_US |
dc.contributor.author | Sandra Reuter | en_US |
dc.contributor.author | The European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group | en_US |
dc.contributor.author | Ana Kaftandzieva | en_US |
dc.contributor.author | Elena Trajkovska-Dokic | en_US |
dc.date.accessioned | 2022-05-05T08:47:17Z | - |
dc.date.available | 2022-05-05T08:47:17Z | - |
dc.date.issued | 2020-10 | - |
dc.identifier.citation | David S, Cohen V, Reuter S, Sheppard AE, Giani T, Parkhill J; European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) Working Group; ESCMID Study Group for Epidemiological Markers (ESGEM), Rossolini GM, Feil EJ, Grundmann H, Aanensen DM. Integrated chromosomal and plasmid sequence analyses reveal diverse modes of carbapenemase gene spread among Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 2020 Oct 6;117(40):25043-25054 | en_US |
dc.identifier.uri | http://hdl.handle.net/20.500.12188/17528 | - |
dc.description.abstract | Molecular and genomic surveillance systems for bacterial pathogens currently rely on tracking clonally evolving lineages. By contrast, plasmids are usually excluded or analyzed with lowresolution techniques, despite being the primary vectors of antibiotic resistance genes across many key pathogens. Here, we used a combination of long- and short-read sequence data of Klebsiella pneumoniae isolates (n = 1,717) from a European survey to perform an integrated, continent-wide study of chromosomal and plasmid diversity. This revealed three contrasting modes of dissemination used by carbapenemase genes, which confer resistance to last-line carbapenems. First, blaOXA-48-like genes have spread primarily via the single epidemic pOXA-48–like plasmid, which emerged recently in clinical settings and spread rapidly to numerous lineages. Second, blaVIM and blaNDM genes have spread via transient associations of many diverse plasmids with numerous lineages. Third, blaKPC genes have transmitted predominantly by stable association with one successful clonal lineage (ST258/512) yet have been mobilized among diverse plasmids within this lineage. We show that these plasmids, which include pKpQIL-like and IncX3 plasmids, have a long association (and are coevolving) with the lineage, although frequent recombination and rearrangement events between them have led to a complex array of mosaic plasmids carrying blaKPC. Taken altogether, these results reveal the diverse trajectories of antibiotic resistance genes in clinical settings, summarized as using one plasmid/multiple lineages, multiple plasmids/multiple lineages, and multiple plasmids/one lineage. Our study provides a framework for the much needed incorporation of plasmid data into genomic surveillance systems, an essential step toward a more comprehensive understanding of resistance spread. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | The Proceedings of the National Academy of Sciences (PNAS) | en_US |
dc.subject | Klebsiella pneumoniae | en_US |
dc.subject | Carbapenem resistance | en_US |
dc.subject | Carbapenemase genes | en_US |
dc.subject | Plasmids | en_US |
dc.subject | Genomics | en_US |
dc.title | Integrated chromosomal and plasmid sequence analyses reveal diverse modes of carbapenemase gene spread among Klebsiella pneumoniae | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1073/pnas.2003407117 | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
Appears in Collections: | Faculty of Medicine: Journal Articles |
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File | Description | Size | Format | |
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PNAS-S. David-2020.pdf | 1.67 MB | Adobe PDF | View/Open |
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